Abstract
Identifying proteins that regulate fin injury is critical to our understanding of regeneration as it relates to both acute wound injury and tissue formation. We have cloned the full-length cDNA of the actinodin4 (and4) gene of Misgurnus anguillicaudatus (MaAnd4) by the RACE method (GenBank Accession No. MG385835). Quantitative RT-PCR analysis during fin regeneration indicated a sudden increase in MaAnd4 expression, with a peak at 3 days post amputation (dpa). In situ analysis showed that MaAnd4 is located in the distal blastema and cells lining the regions of actinotrichia formation at 3 dpa. The highest levels of MaAnd4 expression were observed in the adult testis as well as in the gastrulae during embryonic development. Southern blotting confirmed the existence of and4 in teleosts but not in tetrapods examined. The results show the expression of this gene in actinotrichia formation and its association with fin/limb regeneration ability in teleosts.
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References
Armesto P, Infante C, Cousin X, Ponce M, Manchado M (2015) Molecular and functional characterization of seven Na+/K+-ATPase beta subunit paralogs in Senegalese sole (Solea senegalensis Kaup, 1858). Comp Biochem Physiol B 182:14–26
Brockes JP, Kumar A (2002) Plasticity and reprogramming of differentiated cells in amphibian regeneration. Nat Rev Mol Cell Biol 3:566–574
Dane PJ, Tucker JB (1985) Modulation of epidermal cell shaping and extracellular matrix during caudal fin morphogenesis in the zebrafish Brachydanio rerio. J Embryol Exp Morpholog 87:145–161
Duran I, Csukasi F, Taylor SP, Krakow D, Becerra J, Bombarely A, Marí-Beffa M (2015) Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton. Gene Expr Patterns 19:60–69
Fujimoto T, Kataoka T, Otani S, Saito T, Aita T, Yamaha E, Arai K (2004) Embryonic stages from cleavage to gastrula in the loach Misgurnus anguillicaudatus. Zool Sci 21:747–755
Johnson SL, Bennett P (1999) Growth control in the ontogenetic and regenerating zebrafish fin. Methods Cell Biol 59:301–311
Kujawski S, Lin W, Kitte F, Börmel M, Fuchs S, Arulmozhivarman G, Vogt S, Theil D, Zhang Y, Antos CL (2014) Calcineurin regulates coordinated outgrowth of zebrafish regenerating fins. Dev Cell 28:573–587
Lalonde RL, Moses D, Zhang J, Cornell N, Ekker M, Akimenko MA (2016) Differential actinodin1 regulation in zebrafish and mouse appendages. Dev Biol 417:91–103
Li X, Ma C, Qin YJ, Li YJ, Wu D, Bai LW, Pei AJ (2015) Establishment and characterization of fin cell lines from diploid, triploid, and tetraploid oriental weatherfish (Misgurnus anguillicaudatus). Fish Physiol Biochem 41:661–672
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-delta delta C (T)) method. Methods 25:402–408
McCurley AT, Callard GV (2008) Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment. BMC Mol Boil 9:102
Mercader N (2007) Early steps of paired fin development in zebrafish compared with tetrapod limb development. Develop Growth Differ 49:421–437
Nechiporuk A, Keating MT (2002) A proliferation gradient between proximal and msxb-expressing distal blastema directs zebrafish fin regeneration. Development 129:2607–2617
Nguyen-Chi M, Laplace-Builhé B, Travnickova J et al (2017) TNF signaling and macrophages govern fin regeneration in zebrafish larvae. Cell Death Dis 8:e2979
Nolte H, Hölper S, Housley MP, Islam S, Piller T, Konzer A, Stainier DY, Braun T, Krüger M (2015) Dynamics of zebrafish fin regeneration using a pulsed SILAC approach. Proteomics 15:739–351
Pizette S, Niswander L (1999) BMPs negatively regulate structure and function of the limb apical ectodermal ridge. Development 126:883–894
Smith A, Zhang J, Guay D, Quint E, Johnson A, Akimenko MA (2008) Gene expression analysis on sections of zebrafish regenerating fins reveals limitations in the whole-mount in situ hybridization method. Dev Dyn 237:417–425
Tsonis PA (2000) Regeneration in Vertebrates. Dev Biol 221:273–284
Yano T, Tamura K (2013) The making of differences between fins and limbs. J Anat 222:100–113
Yano T, Abe G, Yokoyama H, Kawakami K, Tamura K (2012) Mechanism of pectoral fin outgrowth in zebrafish development. Development 139:2916–2925
Zhang J, Wagh P, Guay D, Sanchez-Pulido L, Padhi BK, Korzh V, Andrade-Navarro MA, Akimenko MA (2010) Loss of fish actinotrichia proteins and the fin-to-limb transition. Nature 466:234–237
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This research was supported by grants from the National Natural Science Foundation of China (No. U1404330) and Scientific Research Staring Foundation for Ph.D. of Henan Normal University (No. qd15174).
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All procedures performed in studies involving animals were in accordance with the guidelines and ethical standards of China’s Ministry of Science & Technology (Approval Number: 2006-398) and its later amendments or comparable ethical standards.
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Li, L., Xiao, Q., Wang, L. et al. Expression analysis of And4 during fin regeneration in Misgurnus anguillicaudatus provides insights into its function. Fish Physiol Biochem 45, 935–942 (2019). https://doi.org/10.1007/s10695-018-0602-1
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DOI: https://doi.org/10.1007/s10695-018-0602-1