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Molecular cloning of glucose transporter 1 in grouper Epinephelus coioides and effects of an acute hyperglycemia stress on its expression and glucose tolerance

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Abstract

The glucose transporter family proteins play pivotal roles in glucose metabolism. In this study, we successfully cloned the orange spotted grouper (Epinephelus coioides) glucose transporter 1 (EcGlut1) gene (GenBank accession: JQ623903). The full-length EcGlut1 cDNA was 2126 bp with a 1476 bp ORF, a 437bp5′-UTR and 223bp3′-UTR. EcGlut1 is predicted to encode a 491 amino acid protein with a MW of 53.9 kDa, a pI of 8.66 and a Pfam domain. Bioinformatics analysis revealed that EcGlut1 was evolutionally conserved between fishes with 80–89 % amino acid identities. EcGlut1 was expressed predominantly in heart and liver and at lower levels in muscle, intestine, stomach and brain. We also investigated the effect of acute hyperglycemia stress on EcGlut1 expression. In glucose tolerance test, changes in EcGlut1 mRNA expression in response to glucose injection and glucose metabolism-related indictors were assessed at the same time. Glucose injection significantly suppressed EcGlut1 mRNA expression in liver at 12 h and in brain at 24 h postinjection (P < 0.05). EcGlut1 mRNA levels in heart were increased at 6 h (P < 0.05). Plasma glucose level increased significantly and reached its maximum at 3 h postinjection (P < 0.05). The spatiotemporal expression of EcGlut1 and glucose metabolism suggested that orange spotted grouper might rely on fat anabolism to reduce acute hyperglycemia stress and the delayed transcription of EcGlut1 gene might be one reason for glucose intolerance in E. coioides.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31272673), the National Basic Research Program of China (2014CB138600), Guangdong Natural Science Foundation (S2013010016511), Foundation of Guangdong Provincial Key Laboratory of Marine Biotechnology (No. GPKLMB201401), Colleges Pearl River Scholar (GDUPS) (2011) and Project of Enhancing School With Innovation of Guangdong Ocean University (2015KTSCX055). We thank Prof. Ya-Xiong Tao at Auburn University (Auburn, Alabama, USA) for his expert help in editing the English language of an earlier version of this manuscript.

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Authors and Affiliations

  1. Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, People’s Republic of China

    Hongyu Liu, Xiaohui Dong, Shuyan Chi, Qihui Yang, Shuang Zhang & Beiping Tan

  2. Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, People’s Republic of China

    Hongyu Liu

  3. College of Life Sciences, East China Normal University, Shanghai, 200062, People’s Republic of China

    Liqiao Chen

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  1. Hongyu Liu
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  2. Xiaohui Dong
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Correspondence to Beiping Tan.

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Liu, H., Dong, X., Chi, S. et al. Molecular cloning of glucose transporter 1 in grouper Epinephelus coioides and effects of an acute hyperglycemia stress on its expression and glucose tolerance. Fish Physiol Biochem 43, 103–114 (2017). https://doi.org/10.1007/s10695-016-0271-x

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  • DOI: https://doi.org/10.1007/s10695-016-0271-x

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