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Isolation of heat-tolerant myoglobin from Asian swamp eel Monopterus albus

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Abstract

Myoglobin from Asian swamp eel Monopterus albus was purified from fish muscle using salt fractionation followed by column chromatography and molecular filtration. The purified Mb of 0.68 mg/g wet weight of muscle was determined for its molecular mass by MALDI-TOF–MS to be 15,525.18 Da. Using isoelectric focusing technique, the purified Mb showed two derivatives with pI of 6.40 and 7.12. Six peptide fragments of this protein identified by LC–MS/MS were homologous to Mbs of sea raven Hemitripterus americanus, yellowfin tuna Thunnus albacores, blue marlin Makaira nigicans, common carp Cyprinus carpio, and goldfish Carassius auratus. According to the Mb denaturation, the swamp eel Mb had thermal stability higher than walking catfish Clarias batrachus Mb and striped catfish Pangasius hypophthalmus Mb, between 30 and 60 °C. For the thermal stability of Mb, the swamp eel Mb showed a biphasic behavior due to the O2 dissociation and the heme orientation disorder, with the lowest increase in both Kdf and Kds. The thermal sensitivity of swamp eel Mb was lower than those of the other Mbs for both of fast and slow reaction stages. These results suggest that the swamp eel Mb globin structure is thermally stable, which is consistent with heat-tolerant behavior of the swamp eel particularly in drought habitat.

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Acknowledgments

The research funding supported by Center of Excellence for Innovation in Chemistry (PERCH-CIC) and Rajamangala University of Technology Isan, Thailand, was gratefully acknowledged. Thanks were also extended to the Hitachi Scholarship Foundation, Tokyo, Japan.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Center of Excellence for Innovation in Chemistry, Khon Kaen University, 123 Mittrapab Road, T. Ni-Muang, A. Muang, Khon Kaen, 40002, Thailand

    Chatrachatchaya Chotichayapong, Kittipong Wiengsamut & Saksit Chanthai

  2. Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Nison Sattayasai

  3. Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo, 102-8554, Japan

    Toru Tamiya, Nobuyuki Kanzawa & Takahide Tsuchiya

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  1. Chatrachatchaya Chotichayapong
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  2. Kittipong Wiengsamut
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  3. Saksit Chanthai
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  4. Nison Sattayasai
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  5. Toru Tamiya
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  6. Nobuyuki Kanzawa
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  7. Takahide Tsuchiya
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Correspondence to Saksit Chanthai.

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Chotichayapong, C., Wiengsamut, K., Chanthai, S. et al. Isolation of heat-tolerant myoglobin from Asian swamp eel Monopterus albus . Fish Physiol Biochem 38, 1533–1543 (2012). https://doi.org/10.1007/s10695-012-9644-y

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  • DOI: https://doi.org/10.1007/s10695-012-9644-y

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