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Partial characterization of the gene encoding myoadenylate deaminase from the teleost fish Platichthys flesus

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Abstract

AMP-deaminase (AMPD, EC 3.5.4.6), which catalyzes the irreversible hydrolytic deamination of AMP to IMP and ammonia, is an important energy-related enzyme. The partial genomic sequence of the gene encoding myoadenylate deaminase (AMPD1) from the teleost fish Platichthys flesus was determined. The amino acid sequence of P. flesus AMPD1 shows 82% homology with that of the teleost fish Danio rerio. Comparison of genomic sequences of P. flesus and Rattus norvegicus reveals a high degree of conservation of both sequence and structural organization. A phylogenetic analysis of AMPD sequences shows that bony fish and mammalian AMPD1s arise by duplication of a common primordial gene.

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Acknowledgments

We would like to thank M.S. M’Boumba for her excellent technical assistance. We applied the FLAE (first–last-author emphasis) approach combined with EC (equal contribution): + (1st and 2nd authors) for the sequence of authors (Tscharntke et al. 2007).

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Authors and Affiliations

  1. UMR CNRS 6539, Laboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Brest, Place Nicolas Copernic, 29280, Plouzané, France

    M. T. Thébault & A. L. Meistertzheim

  2. UMR CNRS 7144 UPMC Evolution et Génétique des Populations Marines, Station Biologique, Université Européenne de Bretagne, BP 74, Place Georges Teissier, 29682, Roscoff, France

    A. Tanguy

  3. UMR CNRS 6197, Laboratoire de Microbiologie des Environnements Extrêmes, Ifremer, 29280, Plouzané, France

    J. P. Raffin

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  1. M. T. Thébault
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  2. A. Tanguy
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  3. A. L. Meistertzheim
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  4. J. P. Raffin
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Correspondence to M. T. Thébault.

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Thébault, M.T., Tanguy, A., Meistertzheim, A.L. et al. Partial characterization of the gene encoding myoadenylate deaminase from the teleost fish Platichthys flesus . Fish Physiol Biochem 36, 819–825 (2010). https://doi.org/10.1007/s10695-009-9358-y

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  • DOI: https://doi.org/10.1007/s10695-009-9358-y

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