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Pectoral fins of Micropogonias furnieri: a histochemical and ultrastructural study

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Abstract

The myotomal fibres of the pectoral fins of white croaker (Micropogonias furnieri) have been studied using histochemical techniques and transmission electron microscopy (TEM). Succinic dehydrogenase (SDH) for mitochondria, periodic acid Schiff (PAS) for glycogen, Sudan Black for lipids and myosin-adenosintriphosphatase (mATPase) pre-incubated at alkaline and acid pHs were used to visualize the contraction velocity. Three zones were determined: superficial (SZ), medium (MZ) and deep (DZ). Staining for SDH, PAS and Sudan Black was positive only in the SZ. The level of alkaline mATPase was the highest in fibres from the DZ, intermediate in the MZ and low in the SZ; at an acid pH, the reverse was obtained. Fibres from the SZ were small with large quantities of subsarcolemmal mitochondria, scarce intermyofibrilar mitochondria and a well-developed sarcoplasmic reticulum; the myofibrils displayed a polygonal distribution along the entire length of the fibre. Fibres in the MZ were larger than those in the SZ, the myofibrils were densely packed, mitochondria prevailed under the sarcolemma and the sarcoplasmic reticulum was not abundant. Fibres from the DZ were the largest, with ribbon-shaped myofibrils and scarce mitochondria. The intercellular space was abundant and nervous endings were frequently observed.

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Acknowledgements

This research was partly supported by grants from the Universidad Nacional de Mar del Plata (UNMdP) and from the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) Argentina.

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Authors and Affiliations

  1. Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250, 3er piso (7600), Mar del Plata, Buenos Aires, Argentina

    C. V. Devincenti, A. O. Díaz & A. L. Goldemberg

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CP 1033AAJ, Buenos Aires, Argentina

    A. M. García

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  1. C. V. Devincenti
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  2. A. O. Díaz
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  3. A. M. García
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  4. A. L. Goldemberg
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Correspondence to C. V. Devincenti.

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Devincenti, C.V., Díaz, A.O., García, A.M. et al. Pectoral fins of Micropogonias furnieri: a histochemical and ultrastructural study. Fish Physiol Biochem 35, 317–323 (2009). https://doi.org/10.1007/s10695-008-9216-3

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  • DOI: https://doi.org/10.1007/s10695-008-9216-3

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