Abstract
Utilization of resistance (R) genes to breed resistant cultivars is one of the most effective and economical approach to control rice bacterial blight (BB). Xa7, a dominant, broad-spectrum and durable BB-resistant gene, is an ideal gene resource to improve the resistance of rice varieties to bacterial blight, and this well-known gene with important breeding value has been cloned in our recent study. The isolation of Xa7 will facilitate its application in rice breeding by molecular maker-assisted selection (MAS). In this study, based on the specific sequences in the promoter of Xa7, a functional marker, named as MX7, was developed, which can effectively distinguish the dominant BB-resistant Xa7, the recessive BB-susceptible xa7 and the null allele from different rice varieties. Since MX7 is a dominant marker, it can't tell homozygous from heterozygous, a co-dominant marker closely linked to Xa7, named as M6, was developed simultaneously. After verified by amplification in numerous rice varieties and sequence alignment in RICE 3 K database, it is proved that marker M6 is co-segregated with the Xa7 locus. In addition, the effectiveness and accuracy of the two markers were further validated by two F2 populations. Finally, the designed makers were effectively applied in MAS breeding to improve the BB-resistance of a susceptible variety. This study not only provides reliable functional markers for the identification of Xa7 gene in different rice materials, but also will contribute to the application of Xa7 gene in marker-assisted selection to breed rice varieties with durable disease resistance.
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Acknowledgements
We sincerely thank Prof. Zhang Hongsheng (Nanjing Agricultural University) and Gao Zhengyu (China National Rice Research Institute, Chinese Academy of Agricultural Sciences) for kindly providing rice materials.
Funding
This work was supported by the Ministry of Agriculture of China (2016ZX08009003-001), the National Natural Science Foundation of China (32071987, 31871605), and the Natural Science Foundation of Zhejiang Province (LD19C130001).
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JBM and XFC designed and supervised the experiments. PCL, LM and LMH performed the experiments. YLX and YTZ contributed to MAS breeding of Zhe-kang 1. QQ, XMZ and DLZ provided rice varieties and revised the manuscript. CPL, LM and XFC wrote the manuscript with input from all other co-authors. All the authors have read and approved the final manuscript.
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Liu, P., Mei, L., He, L. et al. Development of markers for identification and maker-assisted breeding of Xa7 gene in rice (Oryza sativa L.). Euphytica 217, 134 (2021). https://doi.org/10.1007/s10681-021-02869-x
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DOI: https://doi.org/10.1007/s10681-021-02869-x