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The different origins of artificially-induced unreduced female gametes and their effect on transmitted parental heterozygosity in Populus

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Abstract

The induction of unreduced gametes via chromosome doubling is an effective way to produce triploids. During this process, the transmitted parental heterozygosity varies by different origins of unreduced gametes. In this study, a total of 110 allotriploid individuals from two cross combinations who shared only one male parent were obtained by chromosome doubling of megaspore via high temperature treatment in Populus tomentosa. Twenty-six SSR loci, at which the allelic configurations of the female parents ‘MC1’ and ‘MC2’ were heterozygous and different from male parent ‘YX1’, were screened. Among them, five pairs of pericentromeric SSR primers were selected to identify the origins of unreduced female gametes. Our results showed that 30 individuals from 110 allotriploids derived from the first division restitution (FDR), and 80 allotriploids from the second division restitution (SDR) in these two cross combinations. The transmitted parental heterozygosity of unreduced female gametes via FDR were 0.824 and 0.769 in two cross combinations, respectively, which was significantly higher than that of SDR (0.395 and 0.396, respectively). The FDR type unreduced gametes with higher heterozygosity transmitted from parents may be more desirable in triploid breeding. However, no significant difference in transmitted parental heterozygosity was found between the two FDR groups and two SDR groups. Correct elucidation of the origins of unreduced gametes may be conducive to their effective utilization for further sexual hybridization in triploid poplar breeding.

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Acknowledgements

The authors would like to thank Professor Jun Wang and Professor Pingdong Zhang, College of Biological Sciences and Technology, Beijing Forestry University for precious suggestion before the submission. And the authors would like to thank the staff of Guanxian nursery in Shandong Province, China for providing the experimental field. We are also grateful to the anonymous reviewers and editors for their constructive comments. This research was financially supported by the National Natural Science Foundation of China (No. 31470667).

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Authors and Affiliations

  1. Beijing Advanced Innovation Center for Tree Breeding By Molecular Design, Beijing Forestry University, Beijing, 100083, China

    Xining Geng, Jun Yang, Kang Du, Qiang Han & Xiangyang Kang

  2. National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, China

    Xining Geng, Jun Yang, Kang Du, Qiang Han & Xiangyang Kang

  3. Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China

    Xining Geng, Jun Yang, Kang Du, Qiang Han & Xiangyang Kang

  4. The Laboratory of Forestry Genetics, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China

    Zhiqiang Han

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XG and XK conceived and designed the study. XG and ZH conducted experiments. XG, QH and KD analyzed the data. XK supervised experiments. XG, JY and XK wrote and edited manuscripts. All authors read and approved the manuscript.

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Correspondence to Xiangyang Kang.

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Geng, X., Han, Z., Yang, J. et al. The different origins of artificially-induced unreduced female gametes and their effect on transmitted parental heterozygosity in Populus. Euphytica 215, 181 (2019). https://doi.org/10.1007/s10681-019-2501-7

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