Abstract
Knowledge of the biological properties of pollination and fertilization are essential for breeding and the development of breeding concepts. In this investigation, panmictically cross-pollinated seeds of two selected combining partners of valerian (Valeriana officinalis L.) were produced and in the progeny, the outcrossing rates (OCR) were determined by using amplified fragment length polymorphism analysis (AFLP). The previous assumption that Valeriana officinalis L. shows a predominantly high outcrossing rate (OCR) was confirmed. The OCR ranged from 76.5 to 97.7%. Several mother plants showed an OCR of 100% in their progeny. Partially involved heterozygous DNA-fragments could have led to undetected outcrossings and to a lower OCR. The preferred outcrossing direction and the individual seed amount of the mother plants may influence the performance of a seed mixture, generated by both partners as mother plants.
Similar content being viewed by others
References
Adams WT (1983) Applications of isozymes in tree breeding. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding Part A. Elsevier Science Publishers, Amsterdam, pp 60–64
Aedtner D (2009) Keimfähigkeit und Keimruhe. In: Hoppe B (ed) Handbuch des Arznei- und Gewürzpflanzenanbaus, vol 1. Verein für Arznei- und Gewürzpflanzen SALUPLANTA e.V, Bernburg, pp 646–654
Bomme U (2001) Kulturanleitung für Baldrian. Bayerische Landesanstalt für Landwirtschaft (LfL). http://www.lfl.bayern.de/mam/cms07/ipz/dateien/hugpf_kulturanleitung_f__r_baldrian.pdf. Accessed 13 Nov 2017
Bomme U, Feicht E, Lepschy J (1999) Ergebnisse zu Leistungsprüfungen mit Baldrian- (Valeriana officinalis-) Herkünften. Zeitschrift für Arznei- und Gewürzpflanzen 4:191–197
Carromero W, Hamrick JL (2005) The Mating System of Verbascum thapsus (Scrophulariaceae): the effect of Plant Height. Int J Plant Sci 166:979–983
D’Andrea L, Felber F, Guadagnuolo R (2008) Hybridization rates between lettuce (Lactuca sativa) and its wild relative (L. serriola) under field conditions. Environ Biosaf Res 7:61–71
Dobos G (1996) Fremdbefruchtungsrate und Heterosis bei Mohn. Aktuelle Züchtungsforschung 32:118–120
Duarte-Silva E, Rodrigues LR, Mariath JEA (2011) Contradictory results in pollen viability determination of Valeriana scandens L. Gene Conserv 10:234–242
Ducarme V, Wesselingh RA (2013) Outcrossing rates in two self-compatible, hybridising Rhinanthus species: implication for hybrid formation. Plant Biol 15:541–547
ESCOP (2009) European scientific cooperative on phytotherapy, monograph, valerianae radix, valerian root, 2nd edn. Thieme, Stuttgart
Hartl L, Seefelder S (1998) Diversity of selected hop cultivars detected by fluorescent AFLPs. Theor Appl Genet 96:112–116
Heeger EF (1956) Handbuch des Arznei- und Gewürzpflanzenbaues. Deutscher Bauernverlag, Berlin
Heuberger H, Penzkofer M (2017) Züchterische Verbesserung von Baldrian zur Erhöhung der Rentabilität und Drogenqualität. Bayerische Landesanstalt für Landwirtschaft (LfL). http://www.lfl.bayern.de/ipz/heilpflanzen/168957/index.php. Accessed 13 Nov 2017
Heuberger H, Heubl G, Müller M, Seefelder S, Seidenberger R (2012a) Verwandschaftsverhältnisse und Ploidiestufen ausgewählter Herkünfte Als Ausgangsmaterial für die Züchtung von Arznei-Baldrian (Valeriana officinalis L. s.l.). Zeitschrift für Arznei und Gewürzpflanzen 1:28–37
Heuberger H, Lohwasser U, Schmatz R, Tegtmeier M (2012b) Baldrian (Valeriana officinalis L.). In: Hoppe B (ed) Handbuch des Arznei- und Gewürzpflanzenanbaus Band 4 Arznei- und Gewürzpflanzen A-K. Verein für Arznei- und Gewürzpflanzen SALUPLANTA e.V., Bernburg, pp 164–183
Hoyle M, Cresswell JE (2007) The effect of wind direction on cross-pollination in wind-pollinated GM crops. Ecol Appl 17:1234–1243
ISTA (2009) International Rules for Seed Testing. I. S. Association, Ed. Bassersdorf, Schweiz Kawashima S, Nozaki H, Hamazaki T, Sakata S, Hama T, Matsuo, K, Nagasawa A (2011) Environmental effects on long-range outcrossing rates in maize. Agric Ecosyst Environ 142:410–418
Kempf IM (1986) Grundlagen zur Züchtung von Valeriana officinalis L., Baldrian. Dissertation, Justus-Liebig-University Gießen
Konon NT, Korneva EI (1980) Interspecific crossings of valerian. Rastitel’n Resursy 16:396–400
Konon NT, Novikova NL (1981) Reaction of Valeriana officinalis to inbreeding. Rastitel’n Resursy 17:85–90
Konovalova O, Konon NT, Mikhailova NS, Rybalko KS, Khlaptsev EE (1978) Biology of flowering and pollination in Valeriana officinalis in the Moscow Region. Rastitel’n Resursy 14:73–77
Leach CR, Mayo O, Bürger R (1990) Quantitatively determined self-incompatibility 2. Outcrossing in Borago officinalis. Theor Appl Genet 79:427–430
Muluvi GM, Sprent JI, Odee D, Powell W (2004) Estimates of outcrossing rates in Moringa oleifera using amplified fragment length polymorphism (AFLP). Afr J Biotech 3:146–151
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nabloussi A, Velasco L, Fernandz-Martinez JM (2013) Cross pollination of safflower (Carthamus tinctorius L.) under Moroccan enviromental conditions. Int J Plant Breed 7:145–147
Pathirana R (1994) Natural cross-pollination in sesame (Sesamum indicum L.). Plant Breed 112:167–170
Penzkofer M, Heuberger H, Geyer M, Killermann B, Konnert M (2014a) The application of isozym-polymorphism—a challenge in the breeding of new varieties of valerian (Valeriana officinalis L. sl). Julius-Kühn-Archiv 446:89–93
Penzkofer M, Ziegler E, Heuberger H (2014b) Contents of essential oil, valerenic acids and extractives in different parts of the rootstock of medicinal valerian (Valeriana officinalis L. s.l.). J Appl Res Med Aromat Plants 1:98–106
Penzkofer M, Seefelder S, Heuberger H (2016) Hybrid-breeding of medicinally used valerian (Valeriana officinalis L. s.l.) Apossible concept developing new varieties? In: Marthe F, Riegler H (eds) 6th international symposium breeding research on medicinal and aromatic plants (BREEDMAP 6), Quedlinburg, Germany, June 19–23, 2016, Julius-Kühn-Archive 453
Ph. Eur. 9.1 (2017) European pharmacopoeia. Baldrianwurzel Valerianae radix s.l. 9th ed. Deutscher Apotheker Verlag, Govi-Verlag-Pharmazeutischer Verlag GmbH, Stuttgart, Eschborn
R Core Team (2016) R: A language and environment for statistical computing. Foundation for Statistical Computing, Vienna
Schmalz H (1989) Pflanzenzüchtung, 4th edn. Deutscher Landwirtschaftsverlag, Berlin
Seefelder S, Ermaier H, Schweizer G, Seigner E (2000) Genetic diversity and phylogenetic relationships among accessions of hop, Humulus lupulus, as determined by amplified fragment length polymorphism fingerprinting compared with pedigree data. Plant Breed 119(3):257–263
Shugaeva EV (1979) Male sterility of Valeriana officinalis L. s.l. Sov Genet 15:93–97
Stoskopf NC, Tomes DT, Christie BR (1993) Plant breeding. Theory and practice. Westview Press, Boulder, San Francisco, Oxford
STRATEC (2017) User Manual Invisorb® Spin Plant Mini Kit. https://www.molecular.stratec.com/files/inhalte/Manuals/InvisorbSpinPlantMiniKit_ProtS.pdf. Accessed 28 Sept 2017
Van De Wiel CCM, Lotz LAP (2006) Outcrossing and coexistence of genetically modified with (genetically) unmodified crops: a case study of the situation in the Netherlands. NJAS Wagening J Life Sci 54(1):17–35
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23(21):4407–4414
Wahl S, Plescher A (2014) Saatgutqualität von Kamille (Matricaria recutica L.), Melisse (Melissa officinalis L.) und Baldrian (Valeriana officinalis L.) am deutschen Saatgutmarkt 2009 bis 2011. Zeitschrift für Arznei- und Gewürzpflanzen 19:70–78
Acknowledgements
Special thanks to Dr. Elisabeth Seigner, Dr. Günther Schweizer and Erich Gastl, of the Research Groups IPZ5c, IPZ1b and AVB1 at the Bavarian State Research Center for Agriculture (LfL) for supply, guidance and assistance in the plant cultivation, the seed production and the molecular genetic analysis and to Dr. Fred Eickmeyer (ESKUSA GmbH, Steinach) for the plant cultivation and the seed production.
Funding
The breeding program at the Bavarian State Research Center for Agriculture (LfL) has the objective to improve production profitability of the German medicinal plants production. The project is part of the Demonstration Project Medicinal Plants (KAMEL) and is being supported by Fachagentur Nachwachsende Rohstoffe e.V. (FNR) on behalf of the German Federal Ministry of Food and Agriculture based on a decision of the German Bundestag (FKZ: 22003015, 15NR030).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no competing interests.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Penzkofer, M., Seefelder, S. & Heuberger, H. Estimation of outcrossing rates using genomic marker and determination of seed quality parameters in Valeriana officinalis L. s.l. under field conditions. Euphytica 214, 81 (2018). https://doi.org/10.1007/s10681-018-2164-9
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s10681-018-2164-9