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Genome-wide association mapping of genetic factors controlling Septoria tritici blotch resistance and their associations with plant height and heading date in wheat

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Abstract

Septoria tritici blotch (STB), caused by the ascomycete fungus Zymoseptoria tritici (also known as Mycosphaerella graminicola), is one of the most devastating foliar wheat diseases worldwide. Host resistance is the most effective strategy for management of the disease. A factor that complicates the determination of resistance is its reported interaction with heading date (Hd) and plant height (Ph). In this study, we report findings from a genome-wide association study of resistance to STB in a world-wide collection of 96 wheat accessions. The collection was evaluated under conditions of artificial infection for seedling and adult plant STB resistance, Hd and Ph in field trials. Marker-trait associations (MTAs) were detected using a mixed linear model. STB disease severities showed significant phenotypic variation. In total, 73 MTAs involving STB resistance were detected. The chromosomal locations of some of them were similar to known Stb genes or quantitative trait loci; whereas others were detected in new genomic regions. The field experiment showed evidence of genetic association between STB resistance and Hd, but only for a few genotypes. This was corroborated at the molecular level, where a total of eight genomic regions associated with STB resistance were located in similar positions to MTAs for Hd. New genomic regions associated with STB resistance found here could be useful in wheat breeding aimed at controlling STB after validation in relevant genetic backgrounds

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Acknowledgements

This study was funded by ANPCYT (Agencia Nacional de Promoción Científica y Tecnológica, Argentina) PICT 2181/2010. We wish to thank the staff from the J. Hirschhorn Experimental Station, Faculty of Agricultural and Forestry Sciences, National University of La Plata, Argentina. In addition, we thank Boris Kobiljski, Novi Sad, Serbia for developing and providing the original seed stocks. We also thank to the reviewers and editor for valuable comments and suggestions for improving our manuscript.

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Authors and Affiliations

  1. Faculty of Agriculture and Forestry Sciences, National University of La Plata, 60 and 119. CC 31, 1900, La Plata, Buenos Aires, Argentina

    Guillermo Sebastián Gerard & María Rosa Simón

  2. National Council for Scientific and Technological Research (CONICET), CCT La Plata. 8 N° 1467, 1900, La Plata, Buenos Aires, Argentina

    Guillermo Sebastián Gerard

  3. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstraβe 3, 06466, Stadt Seeland/OT Gatersleben, Germany

    Andreas Börner & Ulrike Lohwasser

  4. Scientific Research Commission (CIC), 526 between 10 and 11, 1900, La Plata, Buenos Aires, Argentina

    María Rosa Simón

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  1. Guillermo Sebastián Gerard
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Correspondence to Guillermo Sebastián Gerard.

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Gerard, G.S., Börner, A., Lohwasser, U. et al. Genome-wide association mapping of genetic factors controlling Septoria tritici blotch resistance and their associations with plant height and heading date in wheat. Euphytica 213, 27 (2017). https://doi.org/10.1007/s10681-016-1820-1

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