Skip to main content
SpringerLink
Log in

Quantitative trait loci underlying the adhesion of Azospirillum brasilense cells to wheat roots

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

The capacity to adhere Azospirillum brasilense cells on the seedling root is a variable trait in wheat varieties (Triticum aestivum L.). The parents of a CIMMYT bread wheat mapping population derived from the cross cv. Opata × synthetic hexaploid line WSHD67.2 (257) contrasted for this trait, providing an opportunity to determine its genetic basis. The capacity to adhere effectively was shown by 32 % of the mapping population individuals. A genetic map was constructed using 157 informative microsatellite loci and 1,356 SNP loci. The resulting quantitative trait loci (QTL) analysis identified four chromosomes as harboring loci associated with adhesion. Chromosome 1A was the site of both a major (LOD >3) and a minor (LOD 2–3) QTL, while the remaining four minor loci mapped to chromosomes 2D, 5A and 6B (two loci). QAdh.uabcs-1A.2 explained 8.6 % of the phenotypic variance and the full set of QTL explained 23.1 %. The source of the positive allele of QAdh.uabcs-1A.2 was cv. Opata. The recognition that adherence has a genetic component has consequences for the use of biofertilizers, and opens the way for breeding for improved levels of A. brasilense adherence.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bashan Y, de-Bashan LE (2010) How the plant growth-promoting bacterium Azospirillum promotes plant growth—a critical assessment. Adv Agron 108:77–136

    CAS  Google Scholar 

  • Bashan Y, Levanony H, Whitmoyer RE (1991) Root surface colonization of non-cereal crop plants by pleomorphic Azospirillum brasilense. Can J Gen Microbiol 137:187–196

    Article  Google Scholar 

  • Castellanos T, Ascencio F, Bashan Y (1997) Cell-surface hydrophobicity and cell-surface charge of Azospirillum spp. FEMS Microbiol Ecol 24:159–172

    Article  CAS  Google Scholar 

  • Castellanos T, Ascencio F, Bashan Y (1998) Cell-surface lectins of Azospirillum spp. Curr Microbiol 36:241–244

    Article  CAS  PubMed  Google Scholar 

  • Cavanagh CR, Chao S, Wang S, Huang BE, Stephen S, Kiani S, Forrest K, Saintenac C, Brown-Guedira GL, Akhunova A, See D, Bai G, Pumphrey M, Tomar L, Wong D, Kong S, Reynolds M, Lopey da Silva M, Bockelman H, Talbert L, Anderson JA, Dreisigacker S, Baenziger S, Carter A, Korzun V, Morrel PL, Dubcovsky J, Morell MK, Sorrels ME, Hayden MJ, Akhunov E (2013) Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proc Natl Acad Sci USA 110:8057–8062

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Compant S, Clément C, Sessitsch A (2010) Plant growth-promoting bacteria in the rhizo- and endosphere of plants: their role, colonization, mechanisms involved and prospects for utilization. Soil Biol Biochem 42:669–678

    Article  CAS  Google Scholar 

  • Díaz-Zorita M, Fernández-Canigia MV (2009) Field performance of a liquid formulation of Azospirillum brasilense on dryland wheat productivity. Eur J Soil Biol 45:3–11

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Ganal MW, Röder MS (2007) Microsatellite and SNP markers in wheat breeding. In: Varshney RK, Tuberosa R (eds) Genomics-assisted crop improvement: genomics applications in crops, vol 2. Springer, Dordrecht, pp 1–24

    Chapter  Google Scholar 

  • Hagen G, Guilfoyle T (2002) Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Mol Biol 49:373–385

    Article  CAS  PubMed  Google Scholar 

  • Hartmann A, Bashan Y (2009) Ecology and application of Azospirillum and other plant growth-promoting bacteria (PGPB). Eur J Soil Biol 45:1–2

    Article  Google Scholar 

  • Kalagudi GM (2010) Mapping of paranodulation response QTL in rice. Jawaharlal Nehru Technological University. http://hdl.handle.net/10603/3463

  • Kawahara Y, de la Bastide M, Hamilton JP, Kanamori H, McCombie WR, Ouyang S, Schwartz DC, Tanaka T, Wu J, Zhou S, Childs KL, Davidson RM, Lin H, Quesada-Ocampo L, Vaillancourt B, Sakai H, Lee SS, Kim J, Numa H, Itoh T, Buell CR, Matsumoto T (2013). Improvement of the Oryza sativa Nipponbare reference genome using next generation sequence and optical map data. Rice 6:4.

  • Michiels KW, Croes CL, Vanderleyden J (1991) Two different modes of attachment of Azospirillum brasilense Sp7 to wheat roots. J Gen Microbiol 137:2241–2246

    Article  CAS  Google Scholar 

  • Millet E, Avivi Y, Feldman M (1984) Yield response of various wheat genotypes to inoculation with Azospirillum brasilense. Plant Soil 80:261–266

    Article  Google Scholar 

  • Mora P, Rosconi F, Franco-Fraguas L, Castro-Sowinski S (2008) Azospirillum brasilense Sp7 produces an outer-membrane lectin that specifically binds to surface-exposed extracellular polysaccharide produced by the bacterium. Arch Microbiol 189:519–524

    Article  CAS  PubMed  Google Scholar 

  • Ng DWK, Wang T, Chandrasekharan MB, Aramayo R, Kertbundit S, Hall TC (2007) Plant SET domain-containing proteins: structure, function and regulation. Biochim et Biophys Acta (BBA)—Gene Struct Expr 1769:316–329

    Article  CAS  Google Scholar 

  • Nosheen A, Bano A, Ullah F, Farooq U, Yasmin H, Hussain I (2011) Effect of plant growth promoting rhizobacteria on root morphology of safflower (Carthamus tinctorius L.). Afr J Biotechnol 10:12639–12649

    Google Scholar 

  • Okon Y, Labandera-Gonzalez CA (1994) Agronomic applications of Azospirillum: an evaluation of 20 years worldwide field inoculation. Soil Biol Biochem 26:1591–1601

    Article  CAS  Google Scholar 

  • Olivares J, Bedmar EJ, Sanjuán J (2013) Biological nitrogen fixation in the context of global change. Mol Plant-Microbe Interact 26:486–494

    Article  CAS  PubMed  Google Scholar 

  • Pothier JF, Wisniewski-Dyé F, Weiss-Gayet M, Moënne-Loccoz Y, Prigent-Combaret C (2007) Promoter-trap identification of wheat seed extract-induced genes in the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp245. Microbiology 153:3608–3622

    Article  CAS  PubMed  Google Scholar 

  • Remans R, Beebe S, Blair M, Manrique G, Tovar E, Rao I, Croonenborghs A, Torres-Gutierrez R, El-Howeity M, Michiels J, Vanderleyden J (2008) Physiological and genetic analysis of root responsiveness to auxin-producing plant growth-promoting bacteria in common bean (Phaseolus vulgaris L.). Plant Soil 302:149–161

    Article  CAS  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed Central  PubMed  Google Scholar 

  • Rodriguez-Sala VM, Nogueira-Cardoso EJB, de Freitas JG, da Silveira AP-D (2007) Wheat genotypes response to inoculation of diazotrophic bacteria in field conditions. Pesq Agropecu Bras 42:833–842

    Article  Google Scholar 

  • Rojas A, Castellanos T, Díaz De León JL (2013) Genetic variation in wheat for Azospirillum brasilense to adhere to the seedling root. Cereal Res Commun 41:275–283

    Article  Google Scholar 

  • Salse J, Abrouk M, Bolot S, Guilhot N, Courcelle E, Faraut T, Waugh R, Close TJ, Messing J, Feuillet C (2009) Reconstruction of monocotelydoneous proto-chromosomes reveals faster evolution in plants than in animals. Proc Natl Acad Sci USA 106:14908–14913

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Schippers B, Bakker AW, Bakker PAHM (1987) Interactions of deleterious and beneficial microorganisms and the effect on cropping practices. Ann Rev Phytopathol 25:339–358

    Article  Google Scholar 

  • Selosse MA, Baudoin E, Vandenkoornhuyse P (2004) Symbiotic microorganisms, a key for ecological success and protection of plants. Comptes Rendus Biologie 327:639–648

    Article  Google Scholar 

  • Smith KP, Handelsman J, Goodman RM (1999) Genetic basis in plants for interactions with disease-suppressive bacteria. Agric Sci 96:4786–4790

    CAS  Google Scholar 

  • Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM (2012) The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion. Plant J 70:978–990

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tien TM, Gaskins MH, Hubell DH (1979) Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L). Appl Environ Microbiol 37:1016–1024

    CAS  PubMed Central  PubMed  Google Scholar 

  • van Ooijen JW (2006) JoinMap ® 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma B.V, Wageningen

    Google Scholar 

  • Vega NOW (2007) A review on beneficial effects of rhizosphere bacteria on soil nutrient availability and plant nutrient uptake. Rev Fac Nal Agr Medellín 60:3621–3643

    Google Scholar 

Download references

Acknowledgments

This research was funded by a grant from CONACYT from the Mexican Government (grant 36608-B) and the bilateral CONACYT-BMBF interchange program. We thank CIMMYT, Texcoco, Mexico, for providing the plant materials and Robert Koebner for language editing.

Author information

Authors and Affiliations

  1. Departamento de Agronomía, Universidad Autónoma de Baja California Sur, La Paz, B.C.S., Mexico

    José Luis Díaz De León & Adriana Rojas-Hernández

  2. Centro de Investigaciones Biológicas del Noroeste (CIBNOR), La Paz, B.C.S., Mexico

    Thelma Castellanos

  3. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

    Jie Ling & Marion S. Röder

Authors
  1. José Luis Díaz De León
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thelma Castellanos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jie Ling
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Adriana Rojas-Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marion S. Röder
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to José Luis Díaz De León.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 14 kb)

Supplementary material 2 (XLSX 108 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Díaz De León, J.L., Castellanos, T., Ling, J. et al. Quantitative trait loci underlying the adhesion of Azospirillum brasilense cells to wheat roots. Euphytica 204, 81–90 (2015). https://doi.org/10.1007/s10681-014-1334-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-014-1334-7

Keywords

Search

Navigation