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QTL analysis for thousand-grain weight under terminal drought stress in bread wheat (Triticum aestivum L.)

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Abstract

Grain yield under post-anthesis drought stress is one of the most complex traits, which is inherited quantitatively. The present study was conducted to identify genes determining post-anthesis drought stress tolerance in bread wheat through Quantitative Trait Loci (QTLs) analysis. Two cultivated bread wheat accessions were selected as parental lines. Population phenotyping was carried out on 133 F2:3 families. Two field experiments and two experiments in the greenhouse were conducted at IPK-Gatersleben, Germany with control and post-anthesis stress conditions in each experiment. Thousand-grain weight was recorded as the main wheat yield component, which is reduced by post-anthesis drought stress. Chemical desiccation was applied in three experiments as simulator of post-anthesis drought stress whereas water stress was applied in one greenhouse experiment. Analysis of variance showed significant differences among the F2:3 families. The molecular genetic linkage map including 293 marker loci associated to 19 wheat chromosomes was applied for QTL analysis. The present study revealed four and six QTLs for thousand-grain weight under control and stress conditions, respectively. Only one QTL on chromosome 4BL was common for both conditions. Five QTLs on chromosomes 1AL, 4AL, 7AS, and 7DS were found to be specific to the stress condition. Both parents contributed alleles for drought tolerance. Taking the known reciprocal translocation of chromosomes 4AL/7BS into account, the importance of the short arms of homoeologous group 7 is confirmed for drought stress.

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Acknowledgments

The first author would like to thank the Iranian government and in particular, the Ministry of Science, Research and Technology of Iran for the financial support. We would like to thank Khaled F. M. Salem for parental lines selection and mapping population development. We are also indebted to Christof Pietsch and Elena K. Khlestkina for their assistance in SSR data scoring and map construction. We would like to appreciate the technical assistance from Mrs. Anette Heber, from Gene and Genome Mapping group and Mrs. Renate Voss, Mrs. Annette Marlow, Mrs. Irma Friese, Mrs. Marion Fischer, from Resources Genetics and Reproduction group from IPK-Gatersleben.

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  1. Khalil Zaynali Nezhad

    Present address: Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, P. O. Box 386, Gorgan, Golestan, Iran

  2. S. Sharma

    Present address: Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan

  3. B. Saal

    Present address: Saatzucht Steinach GmbH, Wittelsbacher Street 15, 94377, Steinach, Germany

Authors and Affiliations

  1. Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Gatersleben, Germany

    Khalil Zaynali Nezhad, M. S. Röder, S. Sharma, U. Lohwasser, R. C. Meyer & A. Börner

  2. Institute of Agricultural and Nutritional Sciences, Martin-Luther-Universität, Halle-Wittenberg, Julius-Kühn-Street 25, 06099, Halle, Germany

    W. E. Weber & B. Saal

  3. Department of Fingerprinting, College of Biotechnology, SVBPUA&T, Modipuram, Meerut, India

    S. Sharma

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  1. Khalil Zaynali Nezhad
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Zaynali Nezhad, K., Weber, W.E., Röder, M.S. et al. QTL analysis for thousand-grain weight under terminal drought stress in bread wheat (Triticum aestivum L.). Euphytica 186, 127–138 (2012). https://doi.org/10.1007/s10681-011-0559-y

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