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A linkage map of cultivated cucumber (Cucumis sativus L.) with 248 microsatellite marker loci and seven genes for horticulturally important traits

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Abstract

A genetic map was developed with microsatellite (simple sequence repeat, SSR) markers and 148 recombinant inbred lines (RILs) derived from a cross between two cultivated cucumber (Cucumis sativus L.) inbred lines 9110Gt and 9930, which was also segregating for seven horticulturally important traits including bitterfree foliage (bi), gynoecious sex expression (F), uniform immature fruit color (u), glossy fruit skin (d), heavy netting of mature fruit (H), no fruit ribbing (fr), and virescent leaf (v-1). Linkage analysis placed 248 microsatellite loci into seven linkage groups spanning 711.9 cM with a mean marker interval of 2.8 cM. Based on shared markers with an early cucumber genetic map, the 7 linkage groups could be assigned to seven cucumber chromosomes. The four fruit epidermal feature-related genes, u, d, H and fr were found to be tightly linked loci in Chromosome 5, and the other three (F, bi and v-1) were placed in different locations of Chromosome 6. It was the first time to map the four genes H, fr, bi and v-1 with molecular markers. In addition, this is the first report of the inheritance of fruit ribbing in cucumber, which was controlled by a single, dominant gene designated as Fr. Mapping information from this study opens the way for marker-assisted selection and map-based cloning of these horticulturally important genes in cucumber.

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Acknowledgments

This research was supported by the Earmark Fund for Modern Agro-industry Technology Research System; the 948 Program (Project # 2008-Z42) of the Ministry of Agriculture, China; the 863 and 973 Program of the Ministry of Science and Technology, China (Projects # 2006AA100108, 2006AA10Z1A8, 2008BADB1B05, and 2009CB119004,); the Key Laboratory of Genetic Improvement for Horticultural Crops, Ministry of Agriculture, China, and Core Research Budget of the Non-profit Governmental Research Institution (ICS, CAAS) (Project # 201011), China.

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Authors and Affiliations

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Han Miao, Shengping Zhang, Xiaowu Wang, Zhonghua Zhang, Man Li, Shengqi Mu, Zhouchao Cheng, Ruowei Zhang, Sanwen Huang, Bingyan Xie, Zhiyuan Fang & Xingfang Gu

  2. College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, China

    Zhenxian Zhang

  3. USDA-ARS Vegetable Crops Research Unit, Horticulture Department, University of Wisconsin, Madison, WI, 53706, USA

    Yiqun Weng

Authors
  1. Han Miao
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  2. Shengping Zhang
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  3. Xiaowu Wang
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  4. Zhonghua Zhang
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  5. Man Li
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  6. Shengqi Mu
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  7. Zhouchao Cheng
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  8. Ruowei Zhang
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  9. Sanwen Huang
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  10. Bingyan Xie
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  11. Zhiyuan Fang
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  12. Zhenxian Zhang
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  13. Yiqun Weng
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  14. Xingfang Gu
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Corresponding author

Correspondence to Xingfang Gu.

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Han Miao and Shengping Zhang contributed equally to this work.

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Miao, H., Zhang, S., Wang, X. et al. A linkage map of cultivated cucumber (Cucumis sativus L.) with 248 microsatellite marker loci and seven genes for horticulturally important traits. Euphytica 182, 167–176 (2011). https://doi.org/10.1007/s10681-011-0410-5

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  • DOI: https://doi.org/10.1007/s10681-011-0410-5

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