Abstract
In several autogamous and vegetatively propagated crops, DNA markers have been used for cultivar identification. However, allogamous crops such as bunching onion (Allium fistulosum L.) are recalcitrant to marker-aided cultivar identification, as well as hybrid seed purity tests, due to the high degree of genetic heterogeneity within each cultivar. To aid cultivar identification and ensure its accuracy in bunching onion, we proposed the “SSR-tagged breeding” scheme in our previous study. The feasibility of this scheme was investigated here using a landrace of bunching onion with two populations tagged with two or four selected SSR markers. Compared with a control population, no significant differences were detected in the agronomic traits of the SSR-tagged populations. The targeted SSR loci were genetically uniform within each population whereas other loci maintained high heterogeneity. These results demonstrate that the SSR-tagged breeding scheme, even with a very small number of markers, is efficient for the identification of newly bred cultivars, and consequently for F1 purity tests, in allogamous crops in which inbreeding depression is as severe as in bunching onion.
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Acknowledgements
We are grateful to Misses K. Tanaka and S. Negoro for their skillful technical assistance. This work was supported by the grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Integrated Research Program for Functionality and Safety of Food Toward an Establishment of Healthy Diet, I-206, Development of evaluation and management methods for supply of safe, II-207, and Research Project for Utilizing Advanced Technology, 1655).
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Tsukazaki, H., Yamashita, KI., Kojima, A. et al. SSR-tagged breeding scheme for allogamous crops: a trial in bunching onion (Allium fistulosum). Euphytica 169, 327–334 (2009). https://doi.org/10.1007/s10681-009-9961-0
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DOI: https://doi.org/10.1007/s10681-009-9961-0