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A genetic linkage map of Populus adenopoda Maxim. × P. alba L. hybrid based on SSR and SRAP markers

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Abstract

Populus adenopoda Maxim. and P. alba L. [section Populus (aspen), genus Populus] are two tree species of ecological and economic value. To date, no high-density genetic maps are available for these two species. In this study, 1100 interspecific hybrids were obtained by controlled crossing and embryo culture. Simple sequence repeat (SSR) and sequence-related amplified polymorphisms (SRAP) were used to genotype 189 F1 individuals. The genetic linkage map of P. adenopoda × P. alba generated from this study includes 212 markers (192 SSRs and 50 SRAPs) and consists of 26 linkage groups spanning 2178.5 cM, with an average distance of 11.7 cM between markers. This is the first SSR- and SRAP-containing genetic linkage map for aspen. The SSRs on the map will serve both as bridges for comparison with the poplar maps published to date and as a direct link to the Populus genomic sequence. Future studies focusing on the data presented here should enhance the density and precision of the map for identifying and localizing quantitative trait loci and promote genomic research on the genus.

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Acknowledgments

We thank the anonymous reviewers for their constructive comments on this manuscript. This work was supported by the National High Technology Research and Development Program of China (863 Program: 2006AA100109) and Key Laboratory of Forest Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education of China (Grant No. FGB200802).

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Authors and Affiliations

  1. Key Laboratory of Forest Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing, 210037, China

    Yuanxiu Wang, Xiaoyan Sun, Biyue Tan, Bo Zhang, Li-an Xu, Minren Huang & Mingxiu Wang

  2. College of Life Science, The Key Laboratory of Molecular Evolution and Biodiversity and the Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Anhui Normal University, Wuhu, 241000, China

    Yuanxiu Wang

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  1. Yuanxiu Wang
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  2. Xiaoyan Sun
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Correspondence to Li-an Xu.

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Wang, Y., Sun, X., Tan, B. et al. A genetic linkage map of Populus adenopoda Maxim. × P. alba L. hybrid based on SSR and SRAP markers. Euphytica 173, 193–205 (2010). https://doi.org/10.1007/s10681-009-0085-3

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