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Estimating genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphisms

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Abstract

Fifteen alfalfa populations consisting of six public cultivars and nine historically recognized sources of alfalfa germplasm in North American cultivars were examined using sequence related amplified polymorphisms (SRAPs). Three bulk DNA samples from each population were evaluated with fourteen different SRAP primer pairs. This resulted in 249 different amplicons, of which over 90% were polymorphic. A dendrogram from the analysis suggests that the public cultivars are quite diverse from all the historical sources of germplasm. The highest mean genetic similarity among the nine original sources of Medicago germplasm was 0.85 between PI 536535 (Peruvian) and 536536 (Indian), while the lowest (0.47) was between PI 560333 (M. falcata) and 536539 (African). The highest mean genetic similarity among the nine original sources of Medicago germplasm and the public alfalfa cultivars was 0.78 between PI 536532 (Ladak) and Vernal, while the lowest (0.59) was between PI 536539 (African) and Oneida. Relationships based on SRAP analysis appear to generally concur with expected relationships based on fall dormancy. This report demonstrates that SRAPs are a promising marker system for detecting polymorphisms in alfalfa.

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Abbreviations

PI:

U.S. National Plant Germplasm System Plant Introduction

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Author notes

  1. T. J. Hughes

    Present address: Department of Plant Pathology, University of Wisconsin, Madison, USA

Authors and Affiliations

  1. USDA-ARS, Vegetable and Forage Crops Research Unit, Prosser, WA, 99350, USA

    G. J. Vandemark, J. J. Ariss, R. C. Larsen & T. J. Hughes

  2. USDA-ARS, Soybean Genomics and Improvement Lab., Beltsville, MD, 20704, USA

    G. A. Bauchan

Authors
  1. G. J. Vandemark
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  2. J. J. Ariss
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  3. G. A. Bauchan
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  4. R. C. Larsen
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  5. T. J. Hughes
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Vandemark, G.J., Ariss, J.J., Bauchan, G.A. et al. Estimating genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphisms. Euphytica 152, 9–16 (2006). https://doi.org/10.1007/s10681-006-9167-7

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  • DOI: https://doi.org/10.1007/s10681-006-9167-7

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