Abstract
Earthworms in general are tolerant to many chemical contaminants including heavy metals and organic pollutants in soil and can bio-accumulate them in their tissues. Earthworms species like Eisenia fetida, Eisenia tetraedra, Lumbricus terrestris, Lumbricus rubellus and Allobophora chlorotica have been found to remove heavy metals (Cd, Pb, Cu, Hg, etc.) pesticides and lipophilic organic micropollutants like the polycyclic aromatic hydrocarbons (PAH) from the soil. They ‘absorb’ the dissolved chemicals through the moist ‘body wall’ in the interstitial water and also ‘ingest’ by mouth while the soil passes through the gut. They either ‘bio-transform’ or ‘biodegrade’ the chemical contaminants rendering them harmless in their bodies. Meanwhile the quality of the soil is improved significantly in terms of physical, chemical and biological properties as the worms thoroughly upturn and disperse the soil, ingest large volumes of soil and excrete nutritive materials (NKP and micronutrients) in the form of ‘vermicasts’ along with millions of beneficial soil microbes including nitrogen fixers.
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References
Achazi RK, Fleener C, Livingstone DR, Peters LD, Schaub K, Schiwe E (1998) Cytochrome P 450 and dependent activity in unexposed and PAH-exposed terrestrial annelids. J Comp Biochem Physiol C 121:339–350
Belfroid A, Meiling J, Drenth HJ, Hermens J, Seinen W, Gestel KV (1995) Dietary uptake of superlipophilic compounds by earthworms (Eisenia andrei). Ecotoxicol Environ Saf 31:185–191
Bhawalkar V, Bhawalkar U (1994) Vermiculture biotechnology. Bhawalkar Earthworm Research Institute (BERI), Pune, India
Binet F, Fayolle L, Pussard M (1998) Significance of earthworms in stimulating soil microbial activity. Biol Fertil Soils 27:79–84
Butt KR (1999) Inoculation of earthworms into reclaimed soils: the UK experience. J Land Degrad Dev 10:565–575
Chaoui HI, Zibilske LM, Ohno T (2003) Effects of earthworms casts and compost on soil microbial activity and plant nutrient availability. Soil Biol Biochem 35(2):295–302
Contreras-Ramos, Silvia M, Alvarez-Bernal, Dioselina & Dendooven Luc (2006) Eisenia fetida increased removal of polycyclic aromatic hydrocarbons (PAHs) from soil. Environ Pollut 141:396–401; Elsevier Pub
Davis B (1971) Laboratory studies on the uptake of dieldrin and DDT by earthworms. Soil Biol Biochem 3:221–223
Edward CA, Lofty JR (1972) Biology of earthworms. Chapman and Hall, London, p 283
Edwards CA, Bohlen PJ (1996) Biology and ecology of earthworms, 3rd edn. Chapman and Hall, London
Edwards CA, Fletcher KE (1988) Interaction between earthworms and microorganisms in organic matter breakdown. Agric Ecosyst Environ 24:235–247
Eijsackers H, Van Gestel CAM, De Jonge S, Muijis B, Slijkerman D (2001) PAH – polluted peat sediments and earthworms: a mutual inference. J Ecotoxicol 10:35–50
Gerard BM (1960) The biology of certain British earthworms in relation to environmental conditions. Ph.D Thesis, University of London
Gunathilagraj K (1996) Earthworm: an introduction; Indian Council of Agricultural Research Training Program. Tamil Nadu Agriculture University, Coimbatore
Hartenstein R, Neuhauser EF, Collier J (1980) Accumulation of heavy metals in the earthworm E. foetida. J Environ Qual 9:23–26
Hand P (1988) Earthworm biotechnology. In: Greenshields R (ed) Resources and application of biotechnology: the new wave. MacMillan Press Ltd., USA
Ireland MP (1979) Metal accumulation by the earthworms Lumbricus rubellus, Dendrobaena veneta and Eiseniella tetraedra living in heavy metal polluted sites. Environ Pollut 19:201–206
Ireland MP (1983) Heavy metals uptake in earthworms; earthworm ecology. Chapman & Hall, London
Jager T, Baerselman R, Dukeman E, De Groot AC, Hogendoorn EA, Jong AD, Kruitbosch J, Peijn W (2003) Availability of PAH to earthworms (Elsenia andrei) in field polluted soil and soil-sediment mixtures. J Environ Toxicol Chem 22(4):767–775
Johnsen AR, Wick LY, Harms H (2005) Principles of microbial PAH degradation in soil. J Environ Pollut 133:71–84
Kanaly RA, Harayama S (2000) Biodegradation of high molecular weight PAHs by bacteria. J Bacteriol 182:2059–2067
Ma WC, Imerzeel J, Bodt J (1995) Earthworm and food interactions on bioaccumulation and disappearance of PAHs: studies on phenanthrene and flouranthene. Ecotoxicol Environ Saf 32:226–232
Malley C, Nair J, Ho G (2006) Impact of heavy metals on enzymatic activity of substrate and on composting worms Eisenia fetida. J Bioresour Technol 97:1498–1502
Markman S, Guschina IA, Barnsleya S, Buchanan KL, Pascoe D, Muller CT (2007) Endocrine disrupting chemicals accumulate in earthworms exposed to sewage effluents; Cardiff School of Biosciences, Cardiff University, Cardiff, U.K. J Chemosphere 70(1):119–125
Morgan M, Burrows I (1982) Earthworms/Microorganisms interactions; Rothamsted Exp. Stn. Rep.
OECD (2000) Guidelines for testing organic chemicals. Proposal for new guidelines: earthworms reproduction tests (E. fetida andrei); Organization for Economic Co-operation and Development (www.oecd.org)
Parish ZD, White JC, Asleyan M, Gent MPN, Lannucci-Berger W, Eitzer BD, Kelsey JW, Mattina MI (2006) Accumulation of weathered PAHs by plant and earthworms species. J Chemosphere 64(4):609–618
Ryan D (2006) Vermiremediation and other bioremediation options for PAHs contaminated soil; Project report submitted for the partial fulfillment of the degree of Bachelor of Environmental Engineering, School of Engineering, Griffith University, Brisbane (Supervisor: Dr. Rajiv Sinha)
Safawat H, Hanna S, Weaver RW (2002) Earthworms survival in oil contaminated soil. J Plant Soil 240:127–132
Satchell JE (1983) Earthworm microbiology. In: Satchel JE (ed) Earthworm ecology—from Darwin to Vermiculture. Chapman and Hall Ltd., London, pp 351–364
Sims RC, Overcash MR (1983) Fate of polynuclear aromatic hydrocarbons (PNAs) in soil-plant systems. Residue Rev 88:2–68
Singleton DR, Hendrix BF, Coleman DC, Whitemann WB (2003) Identification of uncultured bacteria tightly associated with the intestine of the earthworms Lumricus rubellus. Soil Biol Biochem 35:1547–1555
Sinha RK, Herat S, Agarwal S, Asadi R, Carretero E (2002) Vermiculture technology for environmental management: study of action of earthworms Elsinia foetida, Eudrilus euginae and Perionyx excavatus on biodegradation of some community wastes in India and Australia. The Environmentalist, U.K. 22(2):261–268.
Sinha RK, Sinha R (2007) Environmental biotechnology (Role of plants, animals and microbes in environmental management and sustainable development). Aavishkar Publisher, Jaipur, India; ISBN 978-81-7910-229-9; p 315
Tang J, Liste H, Alexander M (2002) Chemical assays of availability to earthworms of PAHs in soil. J Chemosphere 48:35–42
Visvanathan C, Traenklar J, Joseph K, Nagendran R (eds) (2005) Vermicomposting as an eco-tool in sustainable solid waste management; Project report of The Asian Regional Research Program on Environmental Technology (ARRPET); Pub. of Asian Institute of Technology (AIT), Anna University, India; p 36
Acknowledgements
The authors are grateful to Prof. Bofo Yu (HOS of Environmental Engineering and former Director of CESR at Griffith University), Prof. Roger Braddock and Roger Cropp (past and present Directors of CESR), for providing the financial support to carry out the research program. We also thank Jane Giffkins and Michelle in the PC 2 lab for providing the laboratory support and Ehrsam Werner for providing space in the PC 2 lab. Gokul Bharambe deserves special credit for drawing the graphs.
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Sinha, R.K., Bharambe, G. & Ryan, D. Converting wasteland into wonderland by earthworms—a low-cost nature’s technology for soil remediation: a case study of vermiremediation of PAHs contaminated soil. Environmentalist 28, 466–475 (2008). https://doi.org/10.1007/s10669-008-9171-7
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DOI: https://doi.org/10.1007/s10669-008-9171-7