Abstract
Industrial effluents contain hazardous substances that can be a serious threat to the agriculture and human health. In the present study, the cytotoxic and genotoxic impacts of agricultural soil from the industrial area of Dera Bassi (Punjab, India) have been evaluated. Assays such as defects in DNA repair in K-12 mutants of Escherichia coli and chromosomal aberrations in Allium cepa were used to estimate the acute toxicity and chromosomal mutagenesis, respectively. Atomic absorption spectrometry and GC-MS analysis revealed contamination of the soil with high concentrations of heavy metals and organic compounds, respectively. Dichloromethane extract of site I soil sample caused maximum damage to 40 μL mL−1 DNA repair defective mutants and showed 38 and 49% survival in lexA and recA mutants, respectively, which was least among all the sites. In A. cepa test, an inverse relationship between soil extract concentration and the mitotic index was observed. Exposure of growing roots of A. cepa to soil extracts induced chromosomal abnormalities and alterations in mitotic phases in root tip cells. The study concludes that agricultural sites near the industrial area were contaminated with genotoxic and mutagenic compounds. Hence, adequate measures should be taken to reduce the toxicity of industrial effluents discharged onto the agricultural fields.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Abegunrin, T. P., Awe, G. O., Idowu, D. O., & Adejumobi, M. A. (2016). Impact of wastewater irrigation on soil physico-chemical properties, growth and water use pattern of two indigenous vegetables in southwest Nigeria. Catena, 139, 167–178. https://doi.org/10.1016/j.catena.2015.12.014
Aggarwal, M. (2019). Use treated effluents for irrigation with caution, advises CPCB. Mongabay. https://india.mongabay.com/2019/11/use-treated-effluents-for-irrigation-with-caution-advises-cpcb/. Assessed 1 June 2021.
Anh, H. Q., Tomioka, K., Tue, N. M., Tuyen, L. H., Chi, N. K., Minh, T. B., Viet, P. H., & Takahashi, S. (2019). A preliminary investigation of 942 organic micro-pollutants in the atmosphere in waste processing and urban areas, northern Vietnam: levels, potential sources, and risk assessment. Ecotoxicology and Environmental Safety, 167, 354–364.
Augusto-Pinto, L., da Silva, C., Lopes, D., Machado-Silva, A., & Machado, C. (2003). Escherichia coli as a model system to study DNA repair genes of eukaryotic organisms. Genetics and Molecular Research, 2, 77–91.
Chandel, S., Kaur, S., Issa, M., Singh, H. P., Batish, D. R., & Kohli, R. K. (2019). Exposure to mobile phone radiations at 2350 MHz incites cyto- and genotoxic effects in root meristems of Allium cepa. Journal of Environmental Health Science and Engineering, 17, 97–104. https://doi.org/10.1007/s40201-018-00330-1
Cotelle, S., Masfaraud, J. F., & Ferard, J. F. (1999). Assessment of the genotoxicity of contaminated soil with the Allium/Vicia-micronucleus and the Tradescantia-micronucleus assays. Mutatation Research, 426, 167–171. https://doi.org/10.1016/S0027-5107(99)00063-9
Datta, S., Singh, J., Singh, J., Singh, S., & Singh, S. (2018). Assessment of genotoxic effects of pesticide and vermicompost treated soil with Allium cepa test. Sustainable Environment Research, 28, 171–178. https://doi.org/10.1016/j.serj.2018.01.005
da Silva Souza, T., Hencklein, F. A., de Angelis, D. D. F., & Fontanetti, C. S. (2013). Clastogenicity of land farming soil treated with sugar cane vinasse. Environmental Monitoring and Assessment, 185, 1627–1636. https://doi.org/10.1007/s10661-012-2656-3
de Souza, C. P., Guedes Tde, A., & Fontanetti, C. S. (2016). Evaluation of herbicides action on plant bioindicators by genetic biomarkers: a review. Environmental Monitoring and Assessment, 188, 694. https://doi.org/10.1007/s10661-016-5702-8
Dheri, G. S., Brar, M. S., & Malhi, S. S. (2007). Heavy-metal concentration of sewage-contaminated water and its impact on underground water, soil, and crop plants in alluvial soils of northwestern India. Communications in Soil Science and Plant Analysis, 38, 1353–1370. https://doi.org/10.1080/00103620701328743
Dixit, S., Yadav, A., Dwivedi, P. D., & Das, M. (2015). Toxic hazards of leather industry and technologies to combat threat: a review. Journal of Cleaner Production, 87, 39–49. https://doi.org/10.1016/j.jclepro.2014.10.017
Dogra, S. (2018). National Green Tribunal orders shut down of 57 factories in Dera Bassi. Hindustan Times, Mohali. https://bit.ly/3fivQTQ. Accessed 22 June 2021
EPA. (2004). Guidelines for water reuse. United States Environmental Protection Agency. Office of Wastewater Management Municipal Support Division, National Risk Management Research Laboratory (US). Technology Transfer and Support Division.
FAO & UNEP. (2021). Global assessment of soil pollution: Report. Italy.
Fernandez, M. D., Cagigal, E., Vega, M. M., Urzelai, A., Babin, M., Pro, J., & Tarazona, J. V. (2005). Ecological risk assessment of contaminated soils through direct toxicity assessment. Ecotoxicology and Environmental Safety, 62, 174–184. https://doi.org/10.1016/j.ecoenv.2004.11.013
Ho, Y. C., Show, K. Y., Guo, X. X., Norli, I., Alkarkhi Abbas, F. M., & Morad, N. (2012). Industrial discharge and their effect to the environment. In K.-Y. Show (ed.) Industrial Waste. InTech, Shanghai, China. Available from: http://www.intechopen.com/books/industrial-waste/industrial-emissions-and-their-effect-on-the-environment
Husejnovic, M. S., Bergant, M., Jankovic, S., Zizek, S., Smajlovic, A., Softic, A., Music, O., & Antonijevic, B. (2018). Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT). Environmental Geochemistry and Health, 40, 1557–1572. https://doi.org/10.1007/s10653-018-0071-6
Janion, C. (2008). Inducible SOS response system of DNA repair and mutagenesis in Escherichia coli. International Journal of Biological Sciences, 4, 338–344. https://doi.org/10.7150/ijbs.4.338
Jensen, J., & Pedersen, M. B. (2006). Ecological risk assessment of contaminated soil. Reviews of Environmental Contamination and Toxicology, 186, 73–105. https://doi.org/10.1007/0-387-32883-1_3
Kenyon, C. J., & Walker, G. C. (1981). Expression of E. coli uvrA gene is inducible. Nature, 289, 808–810. https://doi.org/10.1038/289808a0
Khan, S., Anas, M., & Malik, A. (2019). Mutagenicity and genotoxicity evaluation of textile industry wastewater using bacterial and plant bioassays. Toxicology Reports, 6, 193–201. https://doi.org/10.1016/j.toxrep.2019.02.002
Knize, M. G., Takemoto, B. T., Lewis, P. R., & Felton, J. S. (1987). The characterization of the mutagenic activity of soil. Mutation Research, 192, 23–30. https://doi.org/10.1016/0165-7992(87)90121-7
Kumari, V., Yadav, A., Haq, I., Kumar, S., Bharagava, R. N., Singh, S. K., & Raj, A. (2016). Genotoxicity evaluation of tannery effluent treated with newly isolated hexavalent chromium reducing Bacillus cereus. Journal of Environmental Management, 183, 204–211. https://doi.org/10.1016/j.jenvman.2016.08.017
Kuzminov, A. (1999). Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda. Microbiology and Molecular Biology Reviews, 63, 751–813. https://doi.org/10.1128/MMBR.63.4.751-813.1999
Leme, D. M., Grummt, T., Heinze, R., Sehr, A., Renz, S., Reinel, S., de Oliveirac, D. P., Ferraz, E. R., de Marchi, M. R., Machado, M. C., Zocolo, G. J., & Marin-Morales, M. A. (2012). An overview of biodiesel soil pollution: data based on cytotoxicity and genotoxicity assessments. Journal of Hazardous Materials, 199, 343–349. https://doi.org/10.1016/j.jhazmat.2011.11.026
Liang, J., Ning, X. A., Sun, J., Song, J., Lu, J., Cai, H., & Hong, Y. (2018). Toxicity evaluation of textile dyeing effluent and its possible relationship with chemical oxygen demand. Ecotoxicology and Environmental Safety, 166, 56–62. https://doi.org/10.1016/j.ecoenv.2018.08.106
Maron, D., & Ames, B. N. (1983). Revised methods for the Salmonella mutagenicity test. Mutation Research, 113, 173–215. https://doi.org/10.1016/0165-1161(83)90010-9
Masłowska, K., Makiela-Dzbenska, K., & Fijalkowska, I. (2019). The SOS system: a complex and tightly regulated response to DNA damage. Environmental and Molecular Mutagenesis, 60, 368–384. https://doi.org/10.1002/em.22267
Odjadjare, E. E., & Okoh, A. I. (2010). Physicochemical quality of an urban municipal wastewater effluent and its impact on the receiving environment. Environmental Monitoring and Assessment, 170, 383–394. https://doi.org/10.1007/s10661-009-1240-y
Osakwe, S. A. (2012). Effect of cassava processing mill effluent on physical and chemical properties of soils in Abraka and Environs, Delta State, Nigeria. Research Journal of Chemical Sciences, 2, 11.
Pasha, M. K., Aarey, A., Tamkeen, S., & Jahan, P. (2015). Forskolin: genotoxicity assessment in Allium cepa. Mutation Research, 777, 29–32. https://doi.org/10.1016/j.mrgentox.2014.11.005
Raza, W., Lee, J., Raza, N., LuoY, Kim KH., & Yang, J. (2019). Removal of phenolic compounds from industrial wastewater based on membrane-based technologies. Journal of Industrial and Engineering Chemistry, 71, 1–18. https://doi.org/10.1016/j.jiec.2018.11.024
Reddy, S., & Osborne, W. J. (2020). Heavy metal determination and aquatic toxicity evaluation of textile dyes and effluents using Artemia salina. Biocatalysis and Agricultural Biotechnology, 25, 101574. https://doi.org/10.1016/j.bcab.2020.101574
Ronen, A., & Glickman, B. W. (2001). Human DNA repair genes. Environmental and Molecular Mutagenesis, 37, 241–283. https://doi.org/10.1002/em.1033
Scolnick, D. M., & Halazonetis, T. D. (2000). Chfr defines a mitotic stress checkpoint that delays entry into metaphase. Nature, 406, 430–435. https://doi.org/10.1038/35019108
Selmi, S. S., Abdelfattah, T. S., & Mostafa, F. D. (2014). Deregulation of mitosis progression and cytotoxic effect triggered in Allium cepa L. roots by Rubus sancatus Schreber extract. Journal of Life Science, 11, 1047–1058.
Sharma, N., Vaid, U., & Sharma, S. K. (2021). Assessment of groundwater quality for drinking and irrigation purpose using hydrochemical studies in Dera Bassi town and its surrounding agricultural area of Dera Bassi Tehsil of Punjab, India. SN Applied Sciences, 3, 245. https://doi.org/10.1007/s42452-021-04199-y
Simmons, L. A., Foti, J. J., Cohen, C. E., Walker, G. C. (2008). The SOS regulatory network. EcoSal Plus, 3. https://doi.org/10.1128/ecosalplus.5.4.3
Singh, A., Sharma, R. K., Agrawal, M., & Marshall, F. (2009). Effects of wastewater irrigation on physicochemical properties of soil and availability of heavy metals in soil and vegetables. Communications in Soil Science and Plant Analysis, 40, 3469–3490. https://doi.org/10.1080/00103620903327543
Tandon, H. L. S. (1993). Methods of analysis of soils, plants, waters, and fertilizers. Fertilizer Development and Consultation Organization, New Delhi, India.
Tedesco, S. B., & Laughinghouse, H. D., IV. (2012). Bioindicator of genotoxicity: the Allium cepa test. In J. K. Srivastava (Ed.), Environmental Contamination (pp. 137–156). InTech Publisher.
Watanabe, T., Takahashi, K., Konishi, E., Hoshino, Y., Hasei, T., Asanoma, M., Hirayama, T., & Wakabayashi, K. (2008). Mutagenicity of surface soil from residential areas in Kyoto city, Japan, and identification of major mutagens. Mutation Research, 649, 201–212. https://doi.org/10.1016/j.mrgentox.2007.09.002
Xiao, R. Y., Wang, Z., Wang, C. X., Yu, G., & Zhu, Y. G. (2006). Genotoxic risk identification of soil contamination at a major industrialized city in northeast China by a combination ofin vitro and in vivo bioassays. Environmental Science and Technology, 40, 6170–6175. https://doi.org/10.1021/es0607335
Yadav, A., Raj, A., Purchase, D., Ferreira, L. F. R., Saratale, G. D., & Bharagava, R. N. (2019). Phytotoxicity, cytotoxicity and genotoxicity evaluation of organic and inorganic pollutants rich tannery wastewater from a common effluent treatment plant (CETP) in Unnao district, India using Vigna radiata and Allium cepa. Chemosphere, 224, 324–332. https://doi.org/10.1016/j.chemosphere.2019.02.124
Acknowledgements
For the GC-MS analysis in the current study, the authors would like to acknowledge Sophisticated Analytical Instrument Facility (SAIF), Panjab University.
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The funds for undertaking this research were provided by University Grants Commission, New Delhi, India (FM and RP).
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HPS, DRB, and RKK conceived the idea and designed this study. FM, RP, and ASG collected the data. RP and FM did the analysis. FM wrote the first draft. RP and SK edited the earlier versions of the manuscript. HPS, DRB, and RKK edited the final versions of the manuscript. All the authors contributed to the final draft of the manuscript.
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Masood, F., Pandey, R., Singh, H.P. et al. Cytotoxic and genotoxic assessment of agricultural soils from an industrial region. Environ Monit Assess 193, 526 (2021). https://doi.org/10.1007/s10661-021-09289-3
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DOI: https://doi.org/10.1007/s10661-021-09289-3