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Using proteomic analysis to find the proteins involved in resistance against Sclerotinia sclerotiorum in adult Brassica napus

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Abstract

Sclerotinia disease, caused by Sclerotinia sclerotiorum, is one of the most serious plant diseases in China. Research on the mechanism of disease resistance to S. sclerotiorum will help solve control problems. In this study, near-isogenic lines were first used in combination with the proteomic technique. A comparison of protein expression profiles in a susceptible line with those in a resistant line during the interaction of adult Brassica napus with S. sclerotiorum resulted in the identification of 20 important proteins related to disease resistance. Those proteins were then determined to be involved in various functions, including pathogen resistance, antioxidation, and transcription regulation. Our finding showed that some proteins involved in defence—a glycine rich protein (GRP); a trypsin inhibitor protein (TIP); two heat shock proteins (HSPs); and a thiol methyltransferase (TMT)—were upregulated or expressed specially in the resistant B. napus lines. These proteins contribute to ROS (reactive oxygen species) elimination and pathogen-defence in the resistant line, which would help the host defend itself against S. sclerotiorum. As a consequence, the onset of PCD (programmed cell death) was delayed, and the spread of S. sclerotiorum was slowed in the resistant line. Presented results underline the role of specific proteins in the disease process. By building on these results, future research may help determine the genes that are important in conveying resistance to S. sclerotiorum in B. napus.

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Acknowledgments

We would like to thank Dr. Li Shuimin for providing technical assistance with the MALDI–TOF/TOF MS analysis. We are grateful to Dr. Liu Yisong for assisting us with the bioinformatics analysis. This work was supported by the China Postdoctoral Science Foundation (Grant No. 20110490147) and the Special Financial Grant from China Postdoctoral Science Foundation (Grant No. 2012 T50693). This work was also supported by the Foundation of Science and Technology Project of Hunan Province (Grant No. 2011RS4007) and Project supported by Hunan Provincial Natural Science Foundation of China (13JJ2027).

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  1. The Oilseed Crop Research Institute, National Oilseed Crop Improvement Center, Hunan Agricultural University, Changsha, 410128, People’s Republic of China

    Li Wen, Tai-Long Tan, Jia-Bin Shu, Qiu-Ping Zhang, Ming-Zhi Yin & Chun-Yun Guan

  2. Department Food & Biological Engineering, Changsha University of Science and Technology, Changsha, 410114, People’s Republic of China

    Li Wen, Ying Chen, Ying Liu, Zhong-Fang Yang & Jun Tao

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  1. Li Wen
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Wen, L., Tan, TL., Shu, JB. et al. Using proteomic analysis to find the proteins involved in resistance against Sclerotinia sclerotiorum in adult Brassica napus . Eur J Plant Pathol 137, 505–523 (2013). https://doi.org/10.1007/s10658-013-0262-z

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