Abstract
The multiplication kinetics of the Flavescence dorée phytoplasma in broad bean after inoculation by the experimental vector Euscelidius variegatus was determined. The number of phytoplasma cells, measured by quantitative real-time PCR in the aerial parts of the plants, increased exponentially over the time. After 22 to 30 days post inoculation, when symptoms appeared, bacterial growth reached a stationary phase. Whatever the time following inoculation there were no statistical differences between numbers of phytoplasma cells in plants infected by Map-FD1 (FD-CAM05) and Map-FD2 (FD92 and FD-PEY05) genotype strains. On the contrary, temperature had an influence on Flavescence dorée phytoplasma multiplication which was nearly twice as fast in broad beans incubated at 25 °C than in broad beans incubated at 20 °C. At 25 °C, plants expressed symptoms 1 week earlier. In a context of climate change, the consequences of a global warming on the Flavescence dorée epidemics are discussed.
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Acknowledgments
We thank our colleagues Jean-Luc Danet, François Ferrer, Kaelig Guionnaud and Denis Lacaze for maintaining phytoplasma infected plants and insects. We are grateful to Jessika Jean-Jacques for her help in validation of the PCR method. We also thank Sandrine Jagoueix-Eveillard and Cristophe Garcion for critical reading of the manuscript. This research was funded by the “Conseil Interprofessionnel du Vin de Bordeaux”, the Regional Council of Aquitaine and by INRA.
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Salar, P., Charenton, C., Foissac, X. et al. Multiplication kinetics of Flavescence dorée phytoplasma in broad bean. Effect of phytoplasma strain and temperature. Eur J Plant Pathol 135, 371–381 (2013). https://doi.org/10.1007/s10658-012-0093-3
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DOI: https://doi.org/10.1007/s10658-012-0093-3