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Investigation into components of partial disease resistance, determined in vitro, and the concept of types of resistance to Fusarium head blight (FHB) in wheat

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Abstract

This work presents an analysis of the relationship between components of partial disease resistance (PDR) detected using in vitro detached leaf and seed germination assays, inoculated with Microdochium majus, and Fusarium head blight (FHB) resistance to Fusarium graminearum assessed using point inoculation, termed Type II resistance. Relationships between in vitro-determined PDR components and FHB resistance using techniques which inoculate the wheat spike uniformly, termed Type I resistance (incidence and severity), have been reported previously. In this study shorter incubation periods, longer latent periods and shorter lesion lengths in the detached leaf assay and higher germination rates in the seed germination assay were related to greater FHB resistance measured by single point inoculation (Type II), collectively explaining 54% of the variation. Overall the relationships observed for Type II FHB resistance were similar to previous findings for Type I resistances. However, the relative magnitude of effects of the individual PDR components determined in vitro varied between FHB disease resistance parameters. Resistance in seed germination and latent period in the detached leaf assay were more strongly related to resistance assessed by point inoculation (Type II) and severity-Type I as opposed to incubation period which was most strongly related to disease incidence-Type I. The results provide evidence that individual components of partial disease resistance differentially affect aspects of FHB disease progression in the wheat spike. This work supports the view that the current model of types of resistance is an oversimplification of the interacting mechanisms underlying expression of FHB resistance.

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Acknowledgements

Dr. Paul Murphy, North Carolina State University, and the contributors to the 2002 US southern soft red winter wheat FHB screening nursery are gratefully acknowledged for the provision of the multi-site point inoculated (Type II) FHB resistance data.

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  1. Institute of Agri-Food and Land Use, School of Biological Sciences, Queens University Belfast, BT9 5AG, Belfast, UK

    R. A. Browne

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Correspondence to R. A. Browne.

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Browne, R.A. Investigation into components of partial disease resistance, determined in vitro, and the concept of types of resistance to Fusarium head blight (FHB) in wheat. Eur J Plant Pathol 123, 229–234 (2009). https://doi.org/10.1007/s10658-008-9353-7

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