Abstract
Heavy metal pollution of soil has become a public concern worldwide since it threats food safety and human health. Sustainable and environmental-friendly remediation technology is urgently needed. Therefore, we investigated the properties and heavy metal removal ability of Enterobacter asburiae G3 (G3), Enterobacter tabaci I12 (I12), and explored the feasibility of remediation Cd, Pb co-contaminated soil by the combination of G3/I12 and biochar. Our results indicated that both strains are highly resistant to Cd, Pb and maintain plant growth-promoting properties. The removal efficiency of G3 for Cd and Pb were 76.79–99.43%, respectively, while the removal efficiency of I12 for Cd and Pb were 62.57–99.55%, respectively. SEM–EDS and XRD analysis revealed that the morphological and structural changes occurred upon heavy metal exposure, metal precipitates were also detected on cell surface. FTIR analysis indicated that functional groups (–OH, –N–H, –C = O, –C-N, –PO4) were involved in Cd/Pb immobilization. Application of the bacteria, biochar, or their combination decreased the acid-extractable Cd, Pb in soil while increased the residual fractions, meanwhile, the bioavailability of both metal elements declined. Besides, these treatments increased soil enzyme (sucrase, catalase and urease) activity and accelerated pakchoi growth, heavy metal accumulation in pakchoi was depressed upon bacteria and/or biochar application, and a synergistic effect was detected when applying bacteria and biochar together. In BC + G3 and BC + I12 treated plants, the Cd and Pb accumulation decreased by 24.42% and 52.19%, 17.55% and 47.36%, respectively. Overall, our study provides an eco-friendly and promising in situ technology that could be applied in heavy metal remediation.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
This research was financially supported by by the National Natural Science Foundation of China (41807123), Natural Science Foundation of Shaanxi Province (2023-JC-YB-265, 2022NY-078).
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TW: Conceptualization, Methodology, Data curation, Funding acquisition, Writing—original draft. HG: Investigation, Methodology, Software, Data curation. FA, LH, : Methodology, Supervision. JG: Conceptualization, Methodology, Data curation, Editing
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Wei, T., Gao, H., An, F. et al. Performance of heavy metal-immobilizing bacteria combined with biochar on remediation of cadmium and lead co-contaminated soil. Environ Geochem Health 45, 6009–6026 (2023). https://doi.org/10.1007/s10653-023-01605-9
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DOI: https://doi.org/10.1007/s10653-023-01605-9