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Hepatotoxic effects caused by simultaneous exposure to noise and toluene in New Zealand white rabbits: a biochemical and histopathological study

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Abstract

The aim of this experimental study was to investigate hepatotoxicity effects of noise and toluene, and in particular, to study hepatotoxicity effects of simultaneous exposure to noise and toluene by histopathological and biochemical experiments. To experiment hepatotoxicity effects of noise and toluene, 100 dB white noise and 1000 ppm toluene vapors were generated during two consecutive weeks in healthy male New Zealand White rabbits. Non-simultaneous exposure to noise and toluene increased liver enzymes and the serum levels of superoxide dismutase, malondialdehyde, and total antioxidant capacity, and also decreased serum level of glutathione peroxidase. Alanine transaminase, aspartate transaminase, gamma-glutamyl transferase, malondialdehyde, total antioxidant capacity, and superoxide dismutase levels increased by simultaneous exposure to noise and toluene. Furthermore, catalase and alkaline phosphatase level decreased by simultaneous exposure to noise and toluene. The hematoxylin and eosin stain (H&E) experiments indicated significant swelling, lipidosis, eosinophilic cytoplasm, pyknosis, karyorrhexis, and disruption of the cytoplasmic membrane in the liver tissue due to exposure to noise, toluene and simultaneous exposure to them.

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Acknowledgements

This research benefited from a grant from Student Research Committee, Tabriz University of Medical Sciences (No. 59262). Also, Drug Applied Research Center (DARC) of Tabriz University of Medical Sciences provided different experimental facilities. This work was done within the context of a master’s thesis in occupational health engineering by AAM (thesis code: 378).

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Authors and Affiliations

  1. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Amirreza Abouee-Mehrizi & Elnaz Rahimi

  2. Department of Occupational Health Engineering, Faculty of Health, Tabriz University of Medical Sciences, Tabriz, Iran

    Amirreza Abouee-Mehrizi, Yahya Rasoulzadeh & Elnaz Rahimi

  3. Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Ahmad Mehdipour

  4. Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Alireza Alihemmati

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  1. Amirreza Abouee-Mehrizi
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Correspondence to Yahya Rasoulzadeh.

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Abouee-Mehrizi, A., Rasoulzadeh, Y., Mehdipour, A. et al. Hepatotoxic effects caused by simultaneous exposure to noise and toluene in New Zealand white rabbits: a biochemical and histopathological study. Ecotoxicology 30, 154–163 (2021). https://doi.org/10.1007/s10646-020-02288-z

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