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Mitigation of Cd toxicity by Mn in young plants of cacao, evaluated by the proteomic profiles of leaves and roots

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Abstract

Cd is a non-essential metal and highly toxic to plants, animals and humans, even at very low concentrations. Cd has been found in cocoa beans and in their products, as in the case of chocolate. Mn plays an important role in photosynthetic and can interact with Cd and attenuate its toxic effects on plants. The objective of this work was to evaluate the mechanisms of Mn response in the mitigation of Cd toxicity in young plants of the CCN 51 cacao genotype submitted to 0.8 mmol Cd kg−1, 1.6 mmol Mn kg−1 or the combination of 0.4 mmol Cd kg−1 + 0.8 mmol Mn kg−1 soil, together with the control treatment (without addition of Cd and Mn in soil), by means of analysis of changes in the profile of exclusive proteins (EP) and differentially accumulated proteins (DAP). Leaf and root proteins were extracted and quantified from the different treatments, followed by proteomic analysis. About eight DAP and 38 EP were identified in leaves, whereas in roots 43 DAP and 21 EP were identified. Some important proteins induced in the presence of Cd and repressed in the presence of Cd + Mn or vice versa, were ATPases, isoflavone reductase, proteasome and chaperonin. It was concluded that proteins involved in oxidoreduction and defense and stress response processes, in addition to other processes, were induced in the presence of Cd and repressed in the presence of Cd + Mn. This demonstrated that Mn was able to mitigate the toxic effects of Cd on young plants of the CCN 51 cocoa genotype.

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Acknowledgements

I would like to thank the Fundação de Amparo à Pesquisa do Estado da Bahia—FAPESB. The project SCA 58-1245-3-237F and SCA 58-8042-8-014F of USDA-ARS-FUNPAB partially supported the laband greenhouse activities. The second author gratefully acknowledges the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, for the concession of a fellowship of scientific productivity. We thank Marshall Elson for excellent review of this paper.

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  1. Department of Biological Sciences, State University of Santa Cruz, Rodovia Jorge Amado, km 16, Ilhéus, BA, 45662-900, Brazil

    Bruna Rafaela Machado Oliveira, Alex-Alan Furtado de Almeida, Carlos P. Pirovani, Joedson P. Barroso, Carlos H. de C. Neto, Nayara A. Santos, Dário Ahnert & Pedro Antonio O. Mangabeira

  2. USDA-ARS-Beltsville Agricultural Research Center, Beltsville, MD, USA

    Viropax C. Baligar

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  1. Bruna Rafaela Machado Oliveira
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Oliveira, B.R.M., de Almeida, AA.F., Pirovani, C.P. et al. Mitigation of Cd toxicity by Mn in young plants of cacao, evaluated by the proteomic profiles of leaves and roots. Ecotoxicology 29, 340–358 (2020). https://doi.org/10.1007/s10646-020-02178-4

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