Skip to main content
SpringerLink
Log in

Biosurfactant activity, heavy metal tolerance and characterization of Joostella strain A8 from the Mediterranean polychaete Megalomma claparedei (Gravier, 1906)

  • Published:
Ecotoxicology Aims and scope Submit manuscript

Abstract

The effect of heavy metals on the activity of biosurfactants produced by Joostella strain A8 from the polychaete Megalomma claparedei was investigated. Biosurfactant activity was first improved by evaluating the influence of abiotic parameters. Higher E24 indices were achieved at 25 °C in mineral salt medium supplemented with 2 % glucose, 3 % sodium chloride (w/v) and 0.1 % ammonium chloride (w/v). Considerable surface tension reduction was never recorded. Heavy metal tolerance was preliminarily assayed by plate diffusion method resulting in the order of toxicity Cd > Cu > Zn. The activity of biosurfactants was then evaluated in the presence of heavy metals at different concentrations in liquid cultures that were incubated under optimal conditions for biosurfactant activity. The production of stable emulsions resulted generally higher in the presence of metals. These findings suggest that biosurfactant production could represent a bacterial adaptive strategy to defend cells from a stress condition derived from heavy metals in the bulk environment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Amoozadeh E, Malek E, Rashidinejad R, Nabavi S, Karbassi M, Ghayoumi R, Ghorbanzadeh-Zafarani G, Salehi H, Sures B (2014) Marine organisms as heavy metal bioindicators in the Persian Gulf and the Gulf of Oman. Environ Sci Pollut Res Int 21:2386–2395

    Article  CAS  Google Scholar 

  • Bodour AA, Guerrero-Barajas C, Jiorle BV, Malcomson ME, Paull AK, Somogyi A, Trinh LN, Bates RB, Maier RM (2004) Structure and characterization of flavolipids, a novel class of biosurfactants produced by Flavobacterium sp. strain MTN11. Appl Environ Microbiol 70:114–120

    Article  CAS  Google Scholar 

  • Das P, Mukherjee S, Sen R (2009) Biosurfactant of marine origin exhibiting heavy metal remediation properties. Bioresour Technol 100:4887–4890

    Article  CAS  Google Scholar 

  • De Souza MJ, Nair S, Loka Bharathi PA, Chandramohan D (2006) Metal and antibiotic-resistance in psychrotrophic bacteria from Antarctic marine waters. Ecotoxicology 15:379–384

    Article  Google Scholar 

  • De Troch M, Cnudde C, Willems A, Moens T, Vanreusel A (2010) Bacterial colonization on fecal pellets of harpacticoid copepods and on their diatom food. Microb Ecol 60:581–591

    Article  Google Scholar 

  • Du Z, Zhang W, Xia H, Lü G, Chen G (2010) Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones. Acta Oceanol Sin 29:62–69

    Article  CAS  Google Scholar 

  • Dupont S, Carre-Mlouka A, Domart-Coulon I, Vacelet J, Bourguet-Kondracki ML (2014) Exploring cultivable Bacteria from the prokaryotic community associated with the carnivorous sponge Asbestopluma hypogea. FEMS Microbiol Ecol 88:160–174

    Article  CAS  Google Scholar 

  • Dyksterhouse SE, Gray JP, Herwig RP, Lara JC, Staley JT (1995) Cycloclasticus pugetii gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium from marine sediments. Int J Syst Bacteriol 45:116–123

    Article  CAS  Google Scholar 

  • Frąc M, Oszust C, Lipiec J (2012) Community level physiological profiles (CLPP), characterization and microbial activity of soil amended with dairy sewage sludge. Sensors 12:3253–3268

    Article  Google Scholar 

  • Gutierrez T, Rhodes G, Mishamandani S, Berry D, Whitman WB, Nichols PD, Semple KT, Aitken MD (2014) polycyclic aromatic hydrocarbon degradation of phytoplankton-associated Arenibacter spp. and description of Arenibacter algicola sp. nov., an aromatic hydrocarbon-degrading bacterium. Appl Environ Microbiol 80:618–628

    Article  CAS  Google Scholar 

  • Hameed A, Shahina M, Lin SY, Lai WL, Liu YC, Hsu H, Cheng C, Young CC (2014) Robertkochia marina gen. nov., sp. nov., of the family Flavobacteriaceae, isolated from surface seawater, and emended descriptions of the genera Joostella and Galbibacter. IJSEM 64:533–539

    CAS  Google Scholar 

  • Hara A, Radin NS (1978) Lipid extraction of tissues with a low-toxicity solvent. Anal Biochem 90:420–426

    Article  CAS  Google Scholar 

  • ISO 5509 (2000). Animal and vegetable fats and oils In: Preparation of Methyl Esters of Fatty Acids

  • Jackson SA, Kennedy J, Morrissey JP, O’Gara F, Dobson ADW (2012) Pyrosequencing reveals diverse and distinct sponge-specific microbial communities in sponges from a single geographical location in Irish waters. Microb Ecol 64:105–116

    Article  Google Scholar 

  • Khopade A, Biao R, Liu X, Mahadik K, Zhang L, Kokare C (2012a) Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination 285:198–204

    Article  CAS  Google Scholar 

  • Khopade A, Ren B, Liu XY, Mahadik K, Zhang L, Kokare C (2012b) Production and characterization of biosurfactant from marine Streptomyces species B3. J Colloid Interface Sci 367:311–318

    Article  CAS  Google Scholar 

  • Kim BC, Lee KH, Kim MN, Jung MY, Chang YH, Lee J, Shin KS (2011) Joostella atrarenae sp. nov., a novel member of the Flavobacteriaceae originating from the black sea sand of Jeju Island. Curr Microbiol 62:606–611

    Article  CAS  Google Scholar 

  • Kiran SG, Anto T, Selvin J, Sabarathnam B, Lipton AP (2010) Optimization and characterization of a new lipopeptide biosurfactant produced by marine Brevibacterium aureum MSA13 in solid state culture. Bioresour Technol 101:2389–2396

    Article  Google Scholar 

  • Lakshmipathy TD, Prasad ASA, Kannabiran K (2010) Production of biosurfactant and heavy metal resistance activity of Streptomyces sp. VITDDK3—a novel halo tolerant actinomycetes isolated from saltpan soil. Adv Biol Res 4:108–115

    CAS  Google Scholar 

  • Licata P, Trombetta D, Cristani M, Martino D, Naccari F (2004) Organochlorine compounds and heavy metals in the soft tissue of the mussel Mytilus galloprovincialis collected from Lake Faro (Sicily, Italy). Environ Int 30:805–810

    Article  CAS  Google Scholar 

  • Lo Giudice A, Caruso C, Mangano S, Bruni V, De Domenico M, Michaud L (2012) Marine bacterioplankton diversity and community composition in an Antarctic coastal environment. Microb Ecol 63:210–223

    Article  Google Scholar 

  • Lotfabad TB, Shourian M, Roostaazad R, Najafabadi AR, Adelzadeh MR, Noghabi KA (2009) An efficient biosurfactant-producing bacterium Pseudomonas aeruginosa MR01, isolated from oil excavation areas in south of Iran. Colloids Surf B Biointerfaces 69:183–193

    Article  CAS  Google Scholar 

  • Maneerat S, Bamba T, Harada K, Kobayashi A, Yamada H, Kawai F (2006) A novel crude oil emulsifier excreted in the culture supernatant of a marine bacterium, Myroides sp. strain SM1 Appl. Microbiol Biotechnol 70:254–259

    Article  CAS  Google Scholar 

  • Quan ZX, Xiao YP, Roh SW, Nam YD, Chang HW, Shin KS, Rhee SK, Park YH, Bae JW (2008) Joostella marina gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the East Sea. Int J Syst Evol Microbiol 58:1388–1392

    Article  CAS  Google Scholar 

  • Rizzo C, Michaud L, Hörmann B, Gerçe B, Syldatk C, Hausmann R, De Domenico E, Lo Giudice A (2013) Bacteria associated with Sabellids (Polychaeta: Annelida) as a novel source of surface active compounds. Mar Pollut Bull 70:125–133

    Article  CAS  Google Scholar 

  • Rizzo C, Michaud L, Syldatk C, Hausmann R, De Domenico E, Lo Giudice A (2014) Influence of salinity and temperature on the activity of biosurfactants by polychaete-associated isolates. Environ Sci Pollut Res 21:2988–3004

    Article  CAS  Google Scholar 

  • Rocha CA, Pedregosa AM, Laborda F (2011) Biosurfactant-mediated biodegradation of straight and methyl-branched alkanes by Pseudomonas aeruginosa ATCC 55925. AMB Express 1:9

    Article  Google Scholar 

  • Satpute SK, Bhawsar BD, Dhakephalkar PK, Chopade BA (2008) Assessment of different screening methods for selecting biosurfactant producing marine bacteria. Indian J Mar Sci 37:243–250

    CAS  Google Scholar 

  • Selvin J, Priya SS, Kiran GS, Thangavelu T, Bai NS (2009) Sponge-associated marine bacteria as indicators of heavy metal pollution. Microbiol Res 164:352–363

    Article  CAS  Google Scholar 

  • Singh P, Cameotra SS (2004) Potential applications of microbial surfactants in biomedical sciences. Trends Biotechnol 22:142–146

    Article  CAS  Google Scholar 

  • Stackebrandt E, Chertkov O, Lapidus A, Nolan M, Lucas S, Han C, Cheng J-F, Tapia R, Goodwin LA, Bruce D, Pitluck S, Liolios K, Mavromatis K, Pagani J, Ivanova N, Mikhailova N, Huntemann M, Pati A, Chen A, Palaniappan K, Rohde M, Tindall BJ, Göker M, Woyke T, Detter JC, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Klenk H-P, Kyrpides NC (2013) High-quality-draft genome sequence of the yellow-pigmented flavobacterium Joostella marina type strain (En5T). Stand Gen Sci 8:37–46

    Article  CAS  Google Scholar 

  • Tuleva B, Christova N, Jordanov B, Jordanov B, Nikolova-Damyanova B, Petrov P (2005) Naphthalene degradation and biosurfactant activity by Bacillus cereus 28BN. Z Naturforsch C 60(577):582

    Google Scholar 

  • Velmurugan N, Kalpana D, Cho JY, Lee GH, Park SH, Lee YS (2011) Phylogenetic analysis of culturable marine bacteria in sediments from South Korean Yellow Sea. Microbiology 80:261–272

    Article  CAS  Google Scholar 

  • Yang X, Li X, Zhou Y, Zheng W, Yu C, Zheng T (2014) Novel insights into the algicidal bacterium DH77-1 killing the toxic dinoflagellate Alexandrium tamarense. Sci Total Environ 482–483:116–124

    Article  Google Scholar 

Download references

Acknowledgments

The authors wish to thank Rossana Rando (Department of Environmental Science, Safety, Territory, of Food and Health, University of Messina) for fatty acid analysis, and Giuseppe Sabatino (Department of Earth Sciences and Physics, University of Messina) for SEM analysis.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Biological and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d’Alcontrès 31, 98166, Messina, Italy

    Carmen Rizzo, Luigi Michaud, Marco Graziano, Emilio De Domenico & Angelina Lo Giudice

  2. Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology (KIT), Engler-Bunte-Ring 1, 76131, Karlsruhe, Germany

    Christoph Syldatk

  3. Institute of Food Science and Biotechnology, Section Bioprocess Engineering, University of Hohenheim, 70593, Stuttgart, Germany

    Rudolf Hausmann

  4. Institute for the Coastal Marine Environment, National Research Council (IAMC-CNR), Spianata San Raineri 86, 98122, Messina, Italy

    Angelina Lo Giudice

Authors
  1. Carmen Rizzo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luigi Michaud
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marco Graziano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Emilio De Domenico
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Christoph Syldatk
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rudolf Hausmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Angelina Lo Giudice
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Angelina Lo Giudice.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rizzo, C., Michaud, L., Graziano, M. et al. Biosurfactant activity, heavy metal tolerance and characterization of Joostella strain A8 from the Mediterranean polychaete Megalomma claparedei (Gravier, 1906). Ecotoxicology 24, 1294–1304 (2015). https://doi.org/10.1007/s10646-015-1504-y

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10646-015-1504-y

Keywords

Search

Navigation