Skip to main content
SpringerLink
Log in

Preliminary evaluation of gene expression profiles in liver of mice exposed to Taihu Lake drinking water for 90 days

  • Published:
Ecotoxicology Aims and scope Submit manuscript

Abstract

Differential gene expression profiling was performed via DNA microarray in the liver tissue of Mus musculus mice after exposure to drinking water of Taihu Lake for 90 days. A total of 75 differentially expressed candidate genes (DEGs) were identified (1.5-fold, p ≤ 0.05), among which the expression of 29 genes was up-regulated and that of 46 genes was down-regulated. Most DEGs were involved in biological process based on gene ontology mapping analysis. The drinking water of Taihu Lake significantly influenced the expression of genes related to cell proliferation, apoptosis, amino acid metabolism, development and immune responses. Long-term exposure to the Taihu drinking water may result in increased carcinogenic risk.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Alatise OI, Schrauzer GN (2010) Lead exposure: a contributing cause of the current breast cancer epidemic in Nigerian women. Biol Trace Elem Res 136(2):127–139. doi:10.1007/s12011-010-8608-2

    Article  CAS  Google Scholar 

  • An DZ, Zhang Q, Wu SM, Wei JY, Yang JJ, Dong FT, Yan XZ, Guo CJ (2010) Changes of metabolic profiles in urine after oral administration of quercetin in rats. Food Chem Toxicol 48(6):1521–1527. doi:10.1016/j.fct.2010.03.019

    Article  CAS  Google Scholar 

  • Anonymous, Consortium F (2006) The transcriptional landscape of the mammalian genome (vol 310, 1559, 2005). Science 311(5768):1713

  • Cavill R, Sidhu JK, Kilarski W, Javerzat S, Hagedorn M, Timothy MDE, Bikfalvi A, Keunt HC (2010) A combined metabonomic and transcriptomic approach to investigate metabolism during development in the chick chorioallantoic membrane. J Proteome Res 9(6):3126–3134. doi:10.1021/pr100033t

    Article  CAS  Google Scholar 

  • Coen M, Holmes E, Lindon JC, Nicholson JK (2008) NMR-based metabolic profiling and metabonomic approaches to problems in molecular toxicology. Chem Res Toxicol 21(1):9–27. doi:10.1021/Tx700335d

    Article  CAS  Google Scholar 

  • Degenhardt K, Mathew R, Beaudoin B, Bray K, Anderson D, Chen GH, Mukherjee C, Shi YF, Gelinas C, Fan YJ, Nelson DA, Jin SK, White E (2006) Autophagy promotes tumor cell survival and restricts necrosis, inflammation, and tumorigenesis. Cancer Cell 10(1):51–64. doi:10.1016/j.ccr.2006.06.001

    Article  CAS  Google Scholar 

  • Gatzidou ET, Zira AN, Theocharis SE (2007) Toxicogenomics: a pivotal piece in the puzzle of toxicological research. J Appl Toxicol 27(4):302–309. doi:10.1002/Jat.1248

    Article  CAS  Google Scholar 

  • Hester SD, Nesnow S (2008) Transcriptional responses in thyroid tissues from rats treated with a tumorigenic and a non-tumorigenic triazole conazole fungicide. Toxicol Appl Pharmacol 227(3):357–369. doi:10.1016/j.taap.2007.10.030

    Article  CAS  Google Scholar 

  • Klopper JP, Berenz A, Hays WR, Sharma V, Pugazhenthi U, Janssen J, Singh M, Bissonnette RP, Haugen BR (2008) In vivo and microarray analysis of rexinoid-responsive anaplastic thyroid carcinoma. Clin Cancer Res 14(2):589–596. doi:10.1158/1078-0432.ccr-07-0269

    Article  CAS  Google Scholar 

  • Li Y, Liu SA, Wang C, Li K, Shan YJ, Wang XJ, Sun CH (2010) Novel biomarkers of 3-chloro-1,2-propanediol exposure by ultra performance liquid chromatography/mass spectrometry based metabonomic analysis of rat urine. Chem Res Toxicol 23(6):1012–1017. doi:10.1021/tx900400p

    Article  CAS  Google Scholar 

  • Lu GH, Ji Y, Zhang HZ, Wu H, Qin J, Wang C (2010) Active biomonitoring of complex pollution in Taihu Lake with Carassius auratus. Chemosphere 79(5):588–594. doi:10.1016/j.chemosphere.2010.01.053

    Article  CAS  Google Scholar 

  • Mao GY, Guo XJ, Kang RY, Ren CS, Yang ZP, Sun YS, Zhang CW, Zhang XJ, Zhang HT, Yang W (2010) Prevalence of disability in an arsenic exposure area in Inner Mongolia, China. Chemosphere 80(9):978–981. doi:10.1016/j.chemosphere.2010.05.040

    Article  CAS  Google Scholar 

  • Moreau A, Vilarem MJ, Maurel P, Pascussi JM (2008) Xenoreceptors CAR and PXR activation and consequences on lipid metabolism, glucose homeostasis, and inflammatory response. Mol Pharm 5(1):35–41. doi:10.1021/mp700103m

    Article  CAS  Google Scholar 

  • Morin R, Team MGCP (2006) The status, quality, and expansion of the NIH full-length cDNA project: the mammalian gene collection (MGC) (vol 14, 2121, 2006). Genome Res 16(6):804–804

    Google Scholar 

  • Muhammad S, Shah MT, Khan S (2010) Arsenic health risk assessment in drinking water and source apportionment using multivariate statistical techniques in Kohistan region, northern Pakistan. Food Chem Toxicol 48(10):2855–2864. doi:10.1016/j.fct.2010.07.018

    Article  CAS  Google Scholar 

  • Nakayama T, Hirakawa H, Shibata K, Abe K, Nagayasu T, Taguchi T (2010) Expression of angiopoietin-like 4 in human gastric cancer: ANGPTL4 promotes venous invasion. Oncol Rep 24(3):599–606. doi:10.3892/or_00000897

    Article  CAS  Google Scholar 

  • Nieuwenhuijsen MJ, Smith R, Golfinopoulos S, Best N, Bennett J, Aggazzotti G, Righi E, Fantuzzi G, Bucchini L, Cordier S, Villanueva CM, Moreno V, La Vecchia C, Bosetti C, Vartiainen T, Rautiu R, Toledano M, Iszatt N, Grazuleviciene R, Kogevinas M (2009) Health impacts of long-term exposure to disinfection by-products in drinking water in Europe: HIWATE. J Water Health 7(2):185–207. doi:10.2166/wh.2009.073

    Article  CAS  Google Scholar 

  • Omelchenko A (2009) Lead as an environmental cardiovascular risk factor. Period Biol 111(1):91–98

    CAS  Google Scholar 

  • Pandey MK, Dhawan A, Das M (2006) Induction of p53, P21Waf1, orinithine decorboxylase activity, and DNA damage leading to cell-cycle arrest and apoptosis following topical application of repeated fish fried oil extract to mice. Mol Carcinog 45(11):805–813

    Article  CAS  Google Scholar 

  • Plewa MJ, Simmons JE, Richardson SD, Wagner ED (2010) Mammalian cell cytotoxicity and genotoxicity of the haloacetic acids, a major class of drinking water disinfection by-products. Environ Mol Mutagen 51(8–9):871–878

    Article  CAS  Google Scholar 

  • Qiao M, Huang S, Wang Z (2008) Partitioning characteristics of PAHs between sediment and water in a shallow lake. J Soils Sediments 8(2):69–73. doi:10.1065/oss2008.03.279

    Article  CAS  Google Scholar 

  • Ritter L, Solomon K, Sibley P, Hall K, Keen P, Mattu G, Linton B (2002) Sources, pathways, and relative risks of contaminants in surface water and groundwater: a perspective prepared for the Walkerton inquiry. J Toxicol Environ Health A 65(1):1–142

    Article  CAS  Google Scholar 

  • Schmidt D, Schwalie PC, Ross-Innes CS, Hurtado A, Brown GD, Carroll JS, Flicek P, Odom DT (2010) A CTCF-independent role for cohesin in tissue-specific transcription. Genome Res 20(5):578–588. doi:10.1101/gr.100479.109

    Article  CAS  Google Scholar 

  • Sen B, Mahadevan B, De Marini DM (2007) Transcriptional responses to complex mixtures—a review. Mutat Res Rev Mutat Res 636(1-3):144–177. doi:10.1016/j.mrrev.2007.08.002

    CAS  Google Scholar 

  • Stella C, Beckwith-Hall B, Cloarec O, Holmes E, Lindon JC, Powell J, van der Ouderaa F, Bingham S, Cross AJ, Nicholson JK (2006) Susceptibility of human metabolic phenotypes to dietary modulation. J Proteome Res 5(10):2780–2788. doi:10.1021/pr060265y

    Article  CAS  Google Scholar 

  • USEPA (2009) National primary drinking water regulations. EPA 816-F-09-0004. http://waterepagov/drink/contaminants

  • Van Boxtel AL, Kamstra JH, Cenijn PH, Pieterse B, Wagner MJ, Antink M, Krab K, Van Der Burg B, Marsh G, Brouwer A, Legler J (2008) Microarray analysis reveals a mechanism of phenolic polybrominated diphenylether toxicity in zebrafish. Environ Sci Technol 42(5):1773–1779. doi:10.1021/Es0720863

    Article  Google Scholar 

  • van Hemert MJ, de Steensma HY, van Heusden GPH (2001) 14-3-3 proteins: key regulators of cell division, signalling and apoptosis. BioEssays 23(10):936–946

    Article  Google Scholar 

  • Vesnoushkin GM, Plotnikova NA, Anisimov VN (2007) Melatonin suppresses benz(a)pyrene-induced carcinogenesis in mice. Vopr Onkol (St Petersbg) 53(1):60–65

    Google Scholar 

  • Wei LL, Sun BJ, Song LR, Nie P (2008) Gene expression profiles in liver of zebrafish treated with microcystin-LR. Environ Toxicol Pharmacol 26(1):6–12. doi:10.1016/j.etap.2007.12.007

    Article  CAS  Google Scholar 

  • Wolf DC, Allen JW, George MH, Hester SD, Sun GB, Moore T, Thai SF, Delker D, Winkfield E, Leavitt S, Nelson G, Roop BC, Jones C, Thibodeaux J, Nesnow S (2006) Toxicity profiles in rats treated with tumorigenic and nontumorigenic triazole conazole fungicides: propiconazole, triadimefon, and myclobutanil. Toxicol Pathol 34(7):895–902. doi:10.1080/01926230601047808

    Article  CAS  Google Scholar 

  • Wu B (2009) Gene expression profiles in liver of mouse after chronic exposure to drinking water. J Appl Toxicol. doi:10.1002/jat.1441:9

  • Wu B, Zhang X, Zhang X, Yasun A, Zhang Y, Zhao D, Ford T, Cheng S (2009a) Semi-volatile organic compounds and trace elements in the Yangtze River source of drinking water. Ecotoxicology 18(6):707–714. doi:10.1007/s10646-009-0331-4

    Article  CAS  Google Scholar 

  • Wu B, Zhang Y, Zhao DY, Zhang XX, Kong ZM, Cheng SP (2009b) Gene expression profiles in liver of mouse after chronic exposure to drinking water. J Appl Toxicol 29(7):569–577. doi:10.1002/jat.1441

    Article  CAS  Google Scholar 

  • Wu J, Hu GJ, Wang XY, Li DM, Yu HX, Han XD (2010) The reproductive toxicity of organic compounds extracted from drinking water sources on Sprague dawley rats: an in vitro study. Environ Toxicol 25(3):284–293. doi:10.1002/tox.20498

    Article  CAS  Google Scholar 

  • Xie YX, Liu J, Benbrahim-Tallaa L, Ward JM, Logsdon D, Diwan BA, Waalkes MP (2007) Aberrant DNA methylation and gene expression in livers of newborn mice transplacentally exposed to a hepatocarcinogenic dose of inorganic arsenic. Toxicology 236(1–2):7–15. doi:10.1016/j.tox.2007.03.021

    Article  CAS  Google Scholar 

  • Yum S, Woo S, Kagami Y, Park HS, Ryu JC (2010) Changes in gene expression profile of medaka with acute toxicity of Arochlor 1260, a polychlorinated biphenyl mixture. Comp Biochem Physiol C-Toxicol Pharmacol 151(1):51–56. doi:10.1016/j.cbpc.2009.08.007

    Article  Google Scholar 

  • Zhang Y, Zhao D, Wu B, Hu F, Kong J, Zhang X, Li M, Cui Y, Cheng S (2009) Effects of the Yangtze River source of drinking water on metabolites of Mus musculus. Ecotoxicology 18(6):722–728. doi:10.1007/s10646-009-0338-x

    Article  Google Scholar 

  • Zhang L, Huang HL, Wu K, Wang MW, Wu BY (2010a) Impact of BTG2 expression on proliferation and invasion of gastric cancer cells in vitro. Mol Biol Rep 37(6):2579–2586. doi:10.1007/s11033-009-9777-y

    Article  CAS  Google Scholar 

  • Zhang XX, Sun SL, Zhang Y, Wu B, Zhang ZY, Liu B, Yang LY, Cheng SP (2010b) Toxicity of purified terephthalic acid manufacturing wastewater on reproductive system of male mice (Mus musculus). J Hazard Mater 176(1–3):300–305

    Article  CAS  Google Scholar 

  • Zhao X, Zhang YL, Li SY (2008) Ecological risk assessment of DDT accumulation in aquatic organisms of Taihu Lake, China. Hum Ecol Risk Assess 14(4):819–834. doi:10.1080/10807030802235268

    Article  CAS  Google Scholar 

  • Zhao ZH, Zhang L, Wu JL, Fan CX, Shang JG (2010) Assessment of the potential mutagenicity of organochlorine pesticides (OCPs) in contaminated sediments from Taihu Lake, China. Mutat Res Genet Toxicol Environ Mutagen 696(1):62–68. doi:10.1016/j.mrgentox.2009.12.013

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by Major State Water Pollution Control and Treatment Technique Program of China [2008ZX07528-005] and Social Development Plan of Science and Technology for Jiangsu Province of China [BE2009668].

Author information

Authors and Affiliations

  1. State Key Laboratory of Pollution Control & Resource Reuse and School of the Environment at Nanjing University, Nanjing, 210046, People’s Republic of China

    Yan Zhang, Rui Zhang, Jie Sun, Bing Wu, Xuxiang Zhang & Shupei Cheng

  2. Nanjing Institute of Environmental Science for Ministry of Environmental Protection of China, Nanjing, 210042, People’s Republic of China

    Weixin Li

Authors
  1. Yan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Weixin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jie Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bing Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xuxiang Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shupei Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shupei Cheng.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, Y., Li, W., Zhang, R. et al. Preliminary evaluation of gene expression profiles in liver of mice exposed to Taihu Lake drinking water for 90 days. Ecotoxicology 20, 1071–1077 (2011). https://doi.org/10.1007/s10646-011-0654-9

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10646-011-0654-9

Keywords

Search

Navigation