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Sequential effects of cadmium on genotoxicity and lipoperoxidation in Vicia faba roots

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Abstract

Kinetics of stress responses to Cd exposure (50, 100 and 200 μM) expanding from 12 to 48 h were studied in roots of hydroponically cultivated-Vicia faba seedlings. The heavy metal induced toxicity symptoms and growth arrest of Vicia roots gradually to the Cd concentration and duration of the treatment. The intracellular oxidative stress was evaluated with the H2O2 production. The H2O2 content increased gradually with the sequestered Cd and root growth inhibition. Lipid peroxidation—evidenced by malondialdehyde (MDA) content and Evans blue uptake—and genotoxicity—evidenced by mitotic index (MI) and micronuclei (MCN) values—were concomitantly investigated in root tips. By 12 h, root meristematic cells lost 15% of their mitotic activity under 50 or 100 μM Cd treatment and 50% under 200 μM Cd treatment and led cells with MCN, while the MDA content and Evans blue absorption were not affected. The loss of membrane integrity occurred subsequently by 24 h. The increase in MDA content in root cells treated with 50, 100 and 200 μM Cd was significantly higher than the control. By 48 h, the MDA content increased 134, 178 or 208% in root cells treated with 50, 100 and 200 μM Cd, respectively. The Evans blue absorption was also affected by 24 h in roots when treated with 200 μM Cd and gradually increase by 48 h with the Cd concentration of the treatment. The decrease of mitotic activity triggered by 12 h was even higher by 24 h and the MI reduced to 44, 56 or 80% compared to the control in the three different Cd concentrations tested. The different kinetics of early in vivo physiological and cytogenetic responses to Cd might be relevant to the characterization of its toxicity mechanisms in disrupting primarily the mitosis process.

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Acknowledgment

This work was supported by a grant from ADEME “Bioindicateurs de Qualité des Sols” (PNETOX 2003).

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Authors and Affiliations

  1. Laboratoire de Physiologie et Génétique végétales, UMR PIAF 547-UBP/INRA, Université Blaise Pascal-Clermont II, Campus universitaire des Cézeaux, 24, avenue des Landais, 63177, Aubiere cedex, France

    D. Souguir, G. Ledoigt & Pascale Goupil

  2. Laboratoire de Physiologie et Génétique des plantes à intérêt agronomique, Faculté des Sciences de Bizerte, 7021, Jarzouna, Tunisie

    D. Souguir & E. Ferjani

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  1. D. Souguir
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  2. E. Ferjani
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  3. G. Ledoigt
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  4. Pascale Goupil
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Correspondence to Pascale Goupil.

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Souguir, D., Ferjani, E., Ledoigt, G. et al. Sequential effects of cadmium on genotoxicity and lipoperoxidation in Vicia faba roots. Ecotoxicology 20, 329–336 (2011). https://doi.org/10.1007/s10646-010-0582-0

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  • DOI: https://doi.org/10.1007/s10646-010-0582-0

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