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Genetic diversification and population structure of Barbus cyri De Filippi, 1865 (Teleostei: Cyprinidae) in northern Iran inferred from the mitochondrial D-loop gene sequence

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Abstract

A genetic survey of Barbus cyri populations from two biogeographical endorheic basins (Caspian Sea and Urmia Lake) was carried out using a mitochondrial marker (partial D-loop) in order to ascertain intra- and inter-population genetic diversity, population demography and to address their genetic structure which is the key to conservation action planning. Analyses were conducted on sequences obtained from 68 individuals collected from 10 sampling sites, from two basins. By means of morphological characteristics all specimens collected from the Caspian Sea basin were ascribed to Barbus cyri. Genetic diversity values (h and π) of sampling groups were all different from 0 (in Babolrud River population) to 0.857 (in Kalibar River population). Population connectivity and colonization patterns of the studied area were inferred from an analysis of molecular variance distribution and evolutionary relationships among haplotypes. The results point to different levels of isolation among sampling groups due to ecological and anthropogenic factors and the effect of an artificial barrier on genetic variability and conservation status of the population. Finally, this study confirms the uncertainty associated with systematic classification of Barbus spp. based on morphological characters due to the phenotypic plasticity of the species.

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Acknowledgements

We are pleased to thank, B.W. Coad and H.R. Zamanian for editing the ms; M. Masoudi, H. Mehraban, A. Gholamhosseini, R. Zamanianjejad, S. Vatandoust, A. Joladeh-Roudbal and G.H. Kamran for helping with fish collection in Iran. The research work was funded by Shiraz University and was approved by Ethics Committee of Biology Department of Shiraz University (ECBD-SU- 909821).

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Authors and Affiliations

  1. Ichthyology and Molecular Systematics Lab., Zoology Section, Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran

    Roozbehan Khaefi, Haimd Reza Esmaeili & Mehrgan Ebrahimi

  2. Department of Earth and Environmental Sciences, Palaeontology & Geobiology, Richard-Wagner-Str. 10, 80333, Munich, Germany

    Haimd Reza Esmaeili

  3. Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, IR, Iran

    Mina Hojat Ansari

  4. School of Biological Sciences, Flinders University, Adelaide, South Australia, Australia

    Mehrgan Ebrahimi

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  1. Roozbehan Khaefi
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Correspondence to Haimd Reza Esmaeili.

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Khaefi, R., Esmaeili, H.R., Ansari, M.H. et al. Genetic diversification and population structure of Barbus cyri De Filippi, 1865 (Teleostei: Cyprinidae) in northern Iran inferred from the mitochondrial D-loop gene sequence. Environ Biol Fish 101, 181–192 (2018). https://doi.org/10.1007/s10641-017-0690-2

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