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Down-Regulation of miR-2053 Inhibits the Development and Progression of Esophageal Carcinoma by Targeting Fyn-Related Kinase (FRK)

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Abstract

Background

MicroRNAs (miRNAs) play essential roles in the regulation and pathophysiology of various types of cancers including esophageal carcinoma (ESCA). Increasing numbers of miRNAs have been identified to be important regulators in the progression of ESCA by regulating gene expression. However, functional miRNAs and the underlying mechanisms involved in ESCA need sufficient elucidation.

Aims

In the present study, the function of miR-2053 was investigated in ESCA cells.

Methods

The expression of miR-2053 was detected in four different ESCA cell lines (Eca109, Ec9706, KYSE30, and TE-1 cells) and normal cell line (HEEC) by qRT-PCR. Cell proliferation, migration, and invasion abilities after knockdown of miR-2053 were assessed by CCK-8 assay, scratch assay, and transwell assay, respectively. Cell cycle of ESCA cells was detected by flow cytometric analysis. Expression of proteins in ESCA cells was detected by Western blot analysis.

Results

The results showed that the expression of miR-2053 was remarkably up-regulated in ESCA tissues and cells lines. Down-regulation of miR-2053 markedly inhibited cell proliferation, migration, and invasion and markedly induced cell cycle arrest and cell apoptosis in ESCA cell lines. Fyn-related kinase (FRK) was a target gene of miR-2053. Moreover, down-regulation of miR-2053 mediated the protein kinase B (AKT)/mammalian target of rapamycin and Wnt3a/β-catenin signaling pathway in ESCA cell lines.

Conclusions

Our results together suggest the potential of regulating miR-2053 expression against development and progression of esophageal carcinoma by targeting FRK.

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Authors

Contributions

YZ was involved in supervision, concept, and analysis; BW, LZ, JL, and PH were involved in data collection and analysis; all authors participated in the manuscript preparation.

Corresponding author

Correspondence to Yan Zhang.

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Conflict of interest

The authors declare that they have no competing interests.

Ethics approval and consent to participate

The present study was approved by the Ethics Committee of the Second Hospital of Jilin University. The research has been carried out in accordance with the World Medical Association Declaration of Helsinki. All patients and healthy volunteers provided written informed consent prior to their inclusion within the study.

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Wang, B., Zhang, L., Li, J. et al. Down-Regulation of miR-2053 Inhibits the Development and Progression of Esophageal Carcinoma by Targeting Fyn-Related Kinase (FRK). Dig Dis Sci 65, 2853–2862 (2020). https://doi.org/10.1007/s10620-019-06015-5

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  • DOI: https://doi.org/10.1007/s10620-019-06015-5

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