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Long Noncoding RNA AFAP1-AS1 Promotes Cell Proliferation and Apoptosis of Gastric Cancer Cells via PTEN/p-AKT Pathway

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Abstract

Background

Long noncoding RNA (lncRNA) plays critical roles in both tumor-suppressive and oncogenic pathways in the pathological development and prognosis of cancers.

Aims

This study aimed to explore the expression of lncRNA AFAP1-AS1 and its function in gastric cancer (GC).

Methods

The expression of AFAP1-AS1 was detected in GC tissues and GC cells by quantitative real-time reverse-transcription PCR. A small interfering RNA (siRNA) that targeted AFAP1-AS1 was transfected into cells to inhibit the expression of AFAP1-AS1. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and colony formation assay were performed to examine the cell proliferation of SGC7901 cell transfected with si-AFAP1-AS1. Cell apoptosis was detected by flow cytometry. The protein level of cleaved PARP, Caspase 3, Caspase 9, Caspase 8, Bcl-2, Bax, p-AKT, total-AKT, and PTEN were detected by Western blot.

Results

AFAP1-AS1 was up-regulated in GC tissues and GC cells. AFAP1-AS1 knockdown suppressed cell viability of SGC7901 transfected with si-AFAP1-AS1. The number of apoptotic SGC7901 cell transfected with si-AFAP1-AS1 was increased by 3.4-fold comparing to that of control. The protein level of cleaved PARP, Caspase 3, and Caspase 9 were increased in SGC7901 transfected with si-AFAP1-AS1, as well as the expression of Bax. The protein level of Bcl-2 was decreased. AFAP1-AS1 knockdown decreased the protein level of p-AKT and increased the expression of PTEN in SGC7901 cells.

Conclusions

AFAP1-AS1 was up-regulated in GC cells and regulated the gastric cancer cell proliferation and apoptosis via PTEN/p-AKT pathway.

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Authors and Affiliations

  1. Department of Institute of Traumatic Surgery, Huaihe Hospital, Henan University, Kaifeng, 475000, China

    Jun-qiang Guo & Shi-jie Li

  2. Department of General Surgery, Huaihe Hospital, Henan University, No. 1 Baobei Rd., Kaifeng, 475000, Henan, China

    Guo-xiao Guo

Authors
  1. Jun-qiang Guo
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  2. Shi-jie Li
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  3. Guo-xiao Guo
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Correspondence to Guo-xiao Guo.

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The authors have no actual or potential conflicts of interest to declare.

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Jun-qiang Guo and Shi-jie Li have ontributed equally to this work.

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Guo, Jq., Li, Sj. & Guo, Gx. Long Noncoding RNA AFAP1-AS1 Promotes Cell Proliferation and Apoptosis of Gastric Cancer Cells via PTEN/p-AKT Pathway. Dig Dis Sci 62, 2004–2010 (2017). https://doi.org/10.1007/s10620-017-4584-0

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  • DOI: https://doi.org/10.1007/s10620-017-4584-0

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