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Detection of Differentially Expressed microRNAs in Serum of Pancreatic Ductal Adenocarcinoma Patients: miR-196a Could Be a Potential Marker for Poor Prognosis

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An Erratum to this article was published on 08 September 2010

Abstract

Background

MicroRNAs (miRNAs) have long been established to remain stable in circulation, and dysregulated miRNAs in serum of tumor patients could potentially serve as novel biomarkers.

Aims

To determine whether certain serum miRNAs could represent potential diagnostic and prognostic biomarkers for pancreatic ductal adenocarcinoma (PDAC).

Methods

About 35 patients diagnosed with PDAC at different stages between August 2007 and January 2009 were enrolled in this study. Sera from 15 chronic pancreatitis (CP) patients and 15 healthy individuals were treated as controls. Quantitative real-time polymerase chain reaction assays specific to mature miRNAs were used to quantify the relative levels of those PDAC-associated serum miRNAs.

Results

Of the seven miRNAs detected, three were identified as differentially expressed in PDAC and control groups. miR-21 was able to distinguish PDAC patients from CP (p = 0.033) and healthy subjects (p = 0.001), whereas miR-155 and miR-196a were able to differentiate sera with sick pancreas (PDAC/CP) from normal pancreas (p = 0.0002 and 0.010, respectively). Serum miR-196a expression levels in unresectable PDAC (stages III and IV) patients were significantly higher than those in resectable (stages I and II) patients (p = 0.001). Furthermore, serum miR-196a expression level was found to have a potential value in predicting median survival time of PDAC patients (high-level miR-196a, 6.1 months, (95% CI, 4.49–7.72) versus low-level miR-196a, 12.00 months, (95% CI, 5.92–18.08), p = 0.007).

Conclusions

Serum miR-196a could be a potential noninvasive marker for PDAC prognosis and selection of laparotomy.

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Abbreviations

miRNA:

microRNA

PDAC:

Pancreatic ductal adenocarcinoma

CP:

Chronic pancreatitis

ROC:

Receiver operating characteristic

TNM:

Tumor-node-metastasis

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Acknowledgments

All authors would like to thank Xiaofei Ye, Ph.D. from the Statistics Department of SMMU for data collection and analysis.

Funding support

The project was supported by the China Key Technology R&D Program (Grant No. 2006BAI02A12) and National Nature Science Foundation of China (30971344).

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Corresponding authors

Correspondence to Yongwen Qin or Zhaoshen Li.

Additional information

Xiangyu Kong, Yiqi Du, and Guokun Wang are the co-first authors, and have contributed equally to this work.

An erratum to this article can be found at http://dx.doi.org/10.1007/s10620-010-1410-3

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Kong, X., Du, Y., Wang, G. et al. Detection of Differentially Expressed microRNAs in Serum of Pancreatic Ductal Adenocarcinoma Patients: miR-196a Could Be a Potential Marker for Poor Prognosis. Dig Dis Sci 56, 602–609 (2011). https://doi.org/10.1007/s10620-010-1285-3

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