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Evaluation for Helicobacter pylori in Meckel’s Diverticulum by Using Real-Time PCR

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Abstract

Background

Although Helicobacter pylori (H. pylori) has been identified in heterotopic gastric mucosa of Meckel’s diverticulum, controversial results are reported in the pertinent literature.

Aims

The aim of this study was to evaluate for the presence of H. pylori histologically using hematoxylin–eosin and Toluidine Blue in Meckel’s diverticulum and by real-time TaqMan polymerase chain reaction (PCR) in those with heterotopic gastric mucosa.

Methods

The study included 21 consecutive patients who had undergone resection of Meckel’s diverticulum at our hospital between 1995 and 2007. The paraffin-embedded tissues were retrieved and reviewed for the presence of histological abnormalities and H. pylori-like organisms and for the presence or absence of heterotopic mucosa. H. pylori was sought in those cases that contained heterotopic gastric mucosa using real-time TaqMan PCR to amplify a fragment of the 23S ribosomal RNA (rRNA) gene of H. pylori.

Results

Upon histological examination, heterotopic gastric mucosa was found to be present in 12 cases. H. pylori was not identified in any of the sections examined. A genomic PCR product was also not obtained in real-time PCR study.

Conclusions

We have confirmed that colonization of H. pylori, if it occurs at all, is exceedingly rare in heterotopic gastric mucosa of Meckel’s diverticulum.

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Conflict of Interest Disclosure

The authors declare no conflict of interest. The authors confirm that there is no financial arrangement.

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Authors and Affiliations

  1. Department of Gastroenterology, Gulhane Military Medical Academy, School of Medicine, Ankara, Turkey

    Ahmet Tuzun, Zulfikar Polat, Guldem Kilciler, Ilker Turan & Ahmet Uygun

  2. Department of Microbiology, Gulhane Military Medical Academy, School of Medicine, Ankara, Turkey

    Abdullah Kilic

  3. Department of Pathology, Gulhane Military Medical Academy, School of Medicine, Ankara, Turkey

    Ayhan Ozcan

  4. GATA Gastroenteroloji Bilim Dali, Etlik, 06010, Ankara, Turkey

    Ilker Turan

Authors
  1. Ahmet Tuzun
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  2. Zulfikar Polat
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  3. Guldem Kilciler
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  4. Ilker Turan
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  5. Abdullah Kilic
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Correspondence to Ilker Turan.

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Tuzun, A., Polat, Z., Kilciler, G. et al. Evaluation for Helicobacter pylori in Meckel’s Diverticulum by Using Real-Time PCR. Dig Dis Sci 55, 1969–1974 (2010). https://doi.org/10.1007/s10620-009-0958-2

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  • DOI: https://doi.org/10.1007/s10620-009-0958-2

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