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Elevated Serum Matrix Metalloproteinase-3 and -7 in H. pylori-Related Gastric Cancer Can Be Biomarkers Correlating with a Poor Survival

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Abstract

Background

Helicobacter pylori (H. pylori) infection up-regulates the expression of matrix metalloproteinases (MMPs), which may be involved in chronic inflammation, ulceration, and even cancer development. This study aimed to test if serum levels of MMP-3, -7, and -9 are correlated with different clinical outcomes in H. pylori-infected subjects and if these are predictive of progression to H. pylori-related gastric cancer.

Method

Two hundred one patients, 28 with H. pylori-negative gastritis and 173 with different H. pylori-positive gastrointestinal diseases (46 gastritis, 43 duodenal ulcers, 29 gastric ulcers, and 55 gastric cancers) were assessed for serum MMP-3, -7, and -9 titers by enzyme-linked immunosorbent assay and validated to their correlations with the different clinical features and survival of patients with H. pylori-positive gastric cancer.

Results

Among the H. pylori-infected subjects, gastric cancer patients had higher serum levels of MMP-3 and MMP-7 than those with duodenal ulcer and gastritis (P < 0.05). For gastric cancer patients, concomitant elevated MMP-3 (>14 ng/ml) and MMP-7 (>4.5 ng/ml) independently correlated with lymph node invasion (P < 0.05) and could be predictive to have shorter 2- or 5-year survivals (log rank test, P = 0.006).

Conclusion

Concomitant elevations of MMP-3 and MMP-7 serum levels in the H. pylori-infected gastric cancer patients could serve as potential biomarkers to correlate with poor survival.

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Abbreviations

MMP:

Matrix metalloproteinase

TIMP:

Tissues inhibitors of matrix metalloproteinase

ELISA:

Enzyme-linked immunosorbent assay

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Acknowledgments

This study was supported by grants from the National Science Council (95-2314-B-006-029-MY3) of Taiwan. All authors have no conflicts of interest.

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Authors and Affiliations

  1. Institute of Basic Medical Sciences, National Cheng Kung University Medical Center, Tainan, Taiwan

    Yi-Chun Yeh, Bor-Shyang Sheu & Jiunn-Jong Wu

  2. Institute of Clinical Medicine, National Cheng Kung University Medical Center, Tainan, Taiwan

    Bor-Shyang Sheu & Hsiu-Chi Cheng

  3. Department of Internal Medicine, National Cheng Kung University Medical Center, 138 Sheng Li Road, Tainan, 70428, Taiwan

    Bor-Shyang Sheu & Hsiu-Chi Cheng

  4. Chung Hwa University of Medical Technology, Tainan, Taiwan

    Yen-Lin Wang

  5. Department of Pathology, National Cheng Kung University Medical Center, Tainan, Taiwan

    Hsiao-Bai Yang

  6. Department of Medical Technology, National Cheng Kung University Medical Center, Tainan, Taiwan

    Jiunn-Jong Wu

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  1. Yi-Chun Yeh
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Correspondence to Bor-Shyang Sheu.

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Yeh, YC., Sheu, BS., Cheng, HC. et al. Elevated Serum Matrix Metalloproteinase-3 and -7 in H. pylori-Related Gastric Cancer Can Be Biomarkers Correlating with a Poor Survival. Dig Dis Sci 55, 1649–1657 (2010). https://doi.org/10.1007/s10620-009-0926-x

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  • DOI: https://doi.org/10.1007/s10620-009-0926-x

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