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Effect of Fat Feeding on Pro-oxidant and Anti-oxidant Enzyme Systems in Rat Intestine: Possible Role in the Turnover of Enterocytes

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Abstract

Immature epithelial cells generated in the crypt base undergo differentiation while progressing to the villus tip, where the cells upon apoptosis are detached from the underlying muscular tissue. We previously reported that lipid peroxidation might be involved in the turnover of enterocytes across the crypt–villus axis in rat intestine (Dig Dis Sci 52:1840–1844, 2007). To examine whether long-term feeding of fat with different fatty-acid composition influences this process, in the present study we investigated the effect of feeding fish oil (n − 3) and corn oil (n − 6) polyunsaturated fatty acids on lipid per-oxidation and anti-oxidant systems in different epithelial cell fractions isolated in rat intestine. Feeding fish oil or corn oil markedly enhanced lipid per-oxidation levels of enterocytes throughout villus height compared with control, but there was no difference in the distribution profile of pro- and anti-oxidant enzyme systems and lipid per-oxidation across the crypt–villus axis under these conditions. Analysis of lipid peroxidation levels in different cell fractions revealed that the thiobarbituric acid reactive substance were 9- to 11-fold higher at the villus tip compared with at the crypt base. The activities of glutathione reductase and glutathione-S-transferase were 2- to 5-fold higher in villus tip compared to the crypt region. However, the activities of superoxide dismutase and catalase were 6- to 8-fold high at the crypt base compared with at villus tip cells. Immunocytolocalization of superoxide dismutase showed high staining in crypt base compared with that in villus, tip cells. These findings further suggest that generation of reactive oxygen species in enterocytes across the crypt–villus axis may be involved in turnover of enterocytes across the crypt–villus unit in rat intestine.

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Abbreviations

TBRS:

Thiobarbituric acid reactive substance

GST:

Glutathione-S-transferase

GR:

Glutathione reductase

SOD:

Superoxide dismutase

ROS:

Reactive oxygen species

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Acknowledgment

Aasma Turan is a recipient of a Senior Research Fellowship from the Indian Council of Medical Research, New Delhi, India.

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Authors and Affiliations

  1. Department of Biochemistry, Panjab University, Chandigarh, 160014, India

    Aasma Turan & Akhtar Mahmood

  2. Department of Medicine, University of Illinois, Chicago, IL, 60612, USA

    Ravinder Gill & Pradeep K. Dudeja

  3. Department of Pathology, Government Medical College, Chandigarh, 160032, India

    Harsh Mohan

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  1. Aasma Turan
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  2. Ravinder Gill
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  3. Pradeep K. Dudeja
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Correspondence to Akhtar Mahmood.

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Turan, A., Gill, R., Dudeja, P.K. et al. Effect of Fat Feeding on Pro-oxidant and Anti-oxidant Enzyme Systems in Rat Intestine: Possible Role in the Turnover of Enterocytes. Dig Dis Sci 54, 1229–1236 (2009). https://doi.org/10.1007/s10620-008-0490-9

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