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Electroacupuncture at Acupoint ST-36 Promotes Contractility of Distal Colon via a Cholinergic Pathway in Conscious Rats

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Abstract

Purpose To investigate the effects and possible mechanism of electroacupuncture (EA) at acupoint ST-36 (Zusanli) on the contractility of the distal colon in conscious rats. Methods Rats were randomized into four groups, including the ST-36 group, the sham group, the saline-plus-ST-36 and the atropine-plus-ST-36 group. Needles were inserted into the acupoints for EA. The distal colonic contractility was measured with a manometric catheter inserted into the distal colon. The colonic contractility was recorded for 1 h before EA, 20 min during EA, and 3 h after EA. Saline or atropine was administrated in the saline-plus-ST-36 or atropine-plus-ST-36 group. Results (1) EA at ST-36 significantly increased the contractility of the distal colon. The mean AUC during and after EA were significantly higher than that before EA in the ST-36 group, a 27% increase during EA and 26% after EA. (2) Atropine abolished the accelerating effect of EA at ST-36. The mean AUC remained unchanged during or after EA compared with that before EA in the atropine-plus-ST-36 group. Conclusions EA at ST-36 has a stimulatory effect on the contractility of the distal colon in conscious rats, and the stimulatory effect may be mediated via the cholinergic pathway.

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References

  1. Campbell A (2002) The origins of acupuncture. Acupunct Med 20:141

    Article  PubMed  Google Scholar 

  2. Li Y, Tougas G, Chiverton SG, Hunt RH (1992) The effect of acupuncture on gastrointestinal function and disorders. Am J Gastroenterol 87:1372–1381

    PubMed  CAS  Google Scholar 

  3. Lux G, Hagel J, Backer P, Backer G, Vogl R, Ruppin H, Domschke S, Domschke W (1994) Acupuncture inhibits vagal gastric acid secretion stimulated by sham feeding in healthy subjects. Gut 35:1026–1029

    Article  PubMed  CAS  Google Scholar 

  4. Zhou L, Chey WY (1984) Electric acupuncture stimulates non-parietal secretion of the stomach in dog. Life Sci 34:2233–2238

    Article  PubMed  CAS  Google Scholar 

  5. Ouyang H, Yin J, Wang Z, Pasricha PJ, Chen JD (2002) Electroacupuncture accelerates gastric emptying in association with changes in vagal activity. Am J Physiol Gastrointest Liver Physiol 282:G390–G396

    PubMed  CAS  Google Scholar 

  6. Lin X, Liang J, Ren J, Mu F, Zhang M, Chen JD (1997) Electrical stimulation of acupuncture points enhances gastric myoelectrical activity in humans. Am J Gastroenterol 92:1527–1530

    PubMed  CAS  Google Scholar 

  7. Wu YL, Chen SF, Pan LL (1994) Effect of electroacupuncture on gastric acid secretion and gut hormones. Zhongguo Zhong Xi Yi Jie He Za Zhi 14:709–711

    PubMed  CAS  Google Scholar 

  8. Chan J, Carr I, Mayberry JF (1997) The role of acupuncture in the treatment of irritable bowel syndrome: a pilot study. Hepatogastroenterology 44:1328–1330

    PubMed  CAS  Google Scholar 

  9. Fireman Z, Segal A, Kopelman Y, Sternberg A, Carasso R (2001) Acupuncture treatment for irritable bowel syndrome. A double-blind controlled study. Digestion 64:100–103

    Article  PubMed  CAS  Google Scholar 

  10. Diehl DL (1999) Acupuncture for gastrointestinal and hepatobiliary disorders. J Altern Complement Med 5:27–45

    Article  PubMed  CAS  Google Scholar 

  11. Broide E, Pintov S, Portnoy S, Barg J, Klinowski E, Scapa E (2001) Effectiveness of acupuncture for treatment of childhood constipation. Dig Dis Sci 46:1270–1275

    Article  PubMed  CAS  Google Scholar 

  12. Klauser AG, Rubach A, Bertsche O, Muller-Lissner SA (1993) Body acupuncture: effect on colonic function in chronic constipation. Z Gastroenterol 31:605–608

    PubMed  CAS  Google Scholar 

  13. Zhu Z, Li H, Chen L, Wang G, Kan C (2003) Acupuncture treatment of habitual constipation. J Tradit Chin Med 23:133

    PubMed  Google Scholar 

  14. Anastasi JK, McMahon DJ (2003) Testing strategies to reduce diarrhea in persons with HIV using traditional Chinese medicine: acupuncture and moxibustion. J Assoc Nurses AIDS Care 14:28–40

    Article  PubMed  Google Scholar 

  15. Burgmann T, Rawsthorne P, Bernstein CN (2004) Predictors of alternative and complementary medicine use in inflammatory bowel disease: do measures of conventional health care utilization relate to use? Am J Gastroenterol 99:889–893

    Article  PubMed  Google Scholar 

  16. Su Z (1992) Acupuncture treatment of infantile diarrhea: a report of 1050 cases. J Tradit Chin Med 12:120–121

    PubMed  CAS  Google Scholar 

  17. Tatewaki M, Harris M, Uemura K, Ueno T, Hoshino E, Shiotani A, Pappas TN, Takahashi T (2003) Dual effects of acupuncture on gastric motility in conscious rats. Am J Physiol Regul Integr Comp Physiol 285:R862–R872

    PubMed  CAS  Google Scholar 

  18. Garamszegi M, Javor T, Suto G, Vincze A, Toth G, Mozsik G (1989) Effect of atropine, PGF2 alpha and cimetidine on the beta-carotene induced cytoprotection in ethanol-treated rats. Acta Physiol Hung 73:221–224

    PubMed  CAS  Google Scholar 

  19. Raboin SJ, Gulley S, Henley SC, Chan WC, Esdaile AR, Sayegh AI (2006) Atropine methyl nitrate increases myenteric but not dorsal vagal complex Fos-like immunoreactivity in the rat. Physiol Behav 88:448–452

    Article  PubMed  CAS  Google Scholar 

  20. Tang NM, Dong HW, Wang XM, Tsui ZC, Han JS (1997) Cholecystokinin antisense RNA increases the analgesic effect induced by electroacupuncture or low dose morphine: conversion of low responder rats into high responders. Pain 71:71–80

    Article  PubMed  CAS  Google Scholar 

  21. Iwa M, Matsushima M, Nakade Y, Pappas TN, Fujimiya M, Takahashi T (2006) Electroacupuncture at ST-36 accelerates colonic motility and transit in freely moving conscious rats. Am J Physiol Gastrointest Liver Physiol 290:G285–G292

    Article  PubMed  CAS  Google Scholar 

  22. Ouyang H, Xing J, Chen J (2004) Electroacupuncture restores impaired gastric accommodation in vagotomized dogs. Dig Dis Sci 49:1418–1424

    Article  PubMed  Google Scholar 

  23. Noguchi E, Ohsawa H, Tanaka H, Ikeda H, Aikawa Y (2003) Electro-acupuncture stimulation effects on duodenal motility in anesthetized rats. Jpn J Physiol 53:1–7

    Article  PubMed  Google Scholar 

  24. Luckensmeyer GB, Keast JR (1998) Projections of pelvic autonomic neurons within the lower bowel of the male rat: an anterograde labelling study. Neuroscience 84:263–280

    Article  PubMed  CAS  Google Scholar 

  25. Nagano M, Ishimizu Y, Saitoh S, Okada H, Fukuda H (2004) The defecation reflex in rats: fundamental properties and the reflex center. Auton Neurosci 111:48–56

    Article  PubMed  Google Scholar 

  26. Goyal RK, Hirano I (1996) The enteric nervous system. N Engl J Med 334:1106–1115

    Article  PubMed  CAS  Google Scholar 

  27. Liu JH, Yan J, Yi SX, Chang XR, Lin YP, Hu JM (2004) Effects of electroacupuncture on gastric myoelectric activity and substance P in the dorsal vagal complex of rats. Neurosci Lett 356:99–102

    Article  PubMed  CAS  Google Scholar 

  28. Gao M, Wang M, Li K, He L (1997) Changes of mu opioid receptor binding sites in rat brain following electroacupuncture. Acupunct Electrother Res 22:161–166

    PubMed  CAS  Google Scholar 

  29. Guo HF, Wang XM, Tian JH, Huo YP, Han JS (1997) 2 Hz and 100 Hz electroacupuncture accelerate the expression of genes encoding three opioid peptides in the rat brain. Sheng Li Xue Bao 49:121–127

    PubMed  CAS  Google Scholar 

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Correspondence to Shi Liu.

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Luo, D., Liu, S., Xie, X. et al. Electroacupuncture at Acupoint ST-36 Promotes Contractility of Distal Colon via a Cholinergic Pathway in Conscious Rats. Dig Dis Sci 53, 689–693 (2008). https://doi.org/10.1007/s10620-007-9929-7

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  • DOI: https://doi.org/10.1007/s10620-007-9929-7

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