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Intestinal 5-Hydroxytryptamine and Mast Cell Infiltration in Rat Experimental Colitis

  • Inflammatory Bowel Disease
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Abstract

The present study evaluated the extent of dysfunction of the 5-hydroxytryptaminergic system in inflamed (distal colon) and noninflamed segments (jejunum and ileum) after intrarectal administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS) in the rat. Dexamethasone was administered to control rats and TNBS-treated animals before and for 7 days after TNBS treatment. Tissue levels of 5-hydroxytryptamine (5-HT) in the inflamed colon were higher than in controls. The densitmast cells was also markedly increased. Treatment with dexamethasone attenuated the inflammatory response but did not prevent the increase in colonic 5-HT. In the noninflamed jejunum and terminal ileum, 5-HT tissue levels were markedly increased, but treatment with dexamethasone prevented this increase. It is concluded that the increase in 5-HT levels in the inflamed colon in TNBS-induced colitis may result fmast cell infiltration. In the noninflamed intestine, 5-HT tissue levels were also increased, favoring the view of a generalized mast cell infiltration.

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Authors and Affiliations

  1. Institute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, 4200-319, Porto, Portugal

    F. Magro MD, S. Fraga BS & P. Soares-Da-Silva MD, PhD

  2. Department of Biochemistry (U38/FCT), Faculty of Medicine, University of Porto, 4200-319, Porto, Portugal

    I. Azevedo MD, PhD

  3. Faculty of Medicine, Institute of Pharmacology and Therapeutics, 4200, Porto, Portugal

    P. Soares-Da-Silva MD, PhD

Authors
  1. F. Magro MD
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  2. S. Fraga BS
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  3. I. Azevedo MD, PhD
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  4. P. Soares-Da-Silva MD, PhD
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Corresponding author

Correspondence to P. Soares-Da-Silva MD, PhD.

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Magro, F., Fraga, S., Azevedo, I. et al. Intestinal 5-Hydroxytryptamine and Mast Cell Infiltration in Rat Experimental Colitis. Dig Dis Sci 51, 495–501 (2006). https://doi.org/10.1007/s10620-006-3161-8

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  • DOI: https://doi.org/10.1007/s10620-006-3161-8

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