Abstract
MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progression of breast cancer. Our results indicate that up-regulation of miR-548 significantly inhibits cell proliferation, migration andinvasion, and induces apoptosis in breast cancer cells. Further studies showed that miR-548 could specifically inhibit E2F3 expression. Moreover, rescue test showed that up-regulation of E2F2 could reverse the effect of miR-548 on proliferation, migration, invasion and apoptosis of breast cancer cells. In general, miR-548 could improve the progression of breast cancer. By targeting E2F2, which may make a potential target for the treatment of breast cancer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Akram M, Iqbal M, Daniyal M, Khan AU (2017) Awareness and current knowledge of breast cancer. Biol Res 50(1):33. https://doi.org/10.1186/s40659-017-0140-9
Chang H, Kim N, Park JH et al (2015) Different microRNA expression levels in gastric cancer depending on Helicobacter pylori infection. Gut Liver. 9(2):188–196. https://doi.org/10.5009/gnl13371
Cheng Y, Li Z, Xie J et al (2018) MiRNA-224-5p inhibits autophagy in breast cancer cells via targeting Smad4. Biochem Biophys Res Commun 506(4):793–798. https://doi.org/10.1016/j.bbrc.2018.10.150
Du Y, Zhu J, Chu BF, Yang YP, Zhang SL (2019) MiR-548c-3p suppressed the progression of papillary thyroid carcinoma via inhibition of the HIF1α-mediated VEGF signaling pathway. Eur Rev Med Pharmacol Sci. 23(15):6570–6578. https://doi.org/10.26355/eurrev_201908_18543
Ferlay J, Soerjomataram I, Dikshit R et al (2015) Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 136(5):E359–E386. https://doi.org/10.1002/ijc.29210
Gao Y, Feng B, Lu L et al (2017) MiRNAs and E2F3: a complex network of reciprocal regulations in human cancers. Oncotarget. 8(36):60624–60639. https://doi.org/10.18632/oncotarget.17364
Guo X, Lee S, Cao P (2019) The inhibitive effect of sh-HIF1A-AS2 on the proliferation, invasion, and pathological damage of breast cancer via targeting miR-548c-3p through regulating HIF-1α/VEGF pathway in vitro and vivo. Onco Targets Ther. 12:825–834. https://doi.org/10.2147/ott.s192377
Harbeck N, Gnant M (2017) Breast cancer. Lancet 389(10074):1134–1150. https://doi.org/10.1016/S0140-6736(16)31891-8
Hassan M, Watari H, AbuAlmaaty A, Ohba Y, Sakuragi N (2014) Apoptosis and molecular targeting therapy in cancer. Biomed Res Int 2014:150845. https://doi.org/10.1155/2014/150845
Huang H (2018) Matrix Metalloproteinase-9 (MMP-9) as a cancer biomarker and MMP-9 biosensors: recent advances. Sensors (Basel). 18(10):3249. https://doi.org/10.3390/s18103249
Kalhori V, Törnquist K (2015) MMP2 and MMP9 participate in S1P-induced invasion of follicular ML-1 thyroid cancer cells. Mol Cell Endocrinol 404:113–122. https://doi.org/10.1016/j.mce.2015.01.037
Katayama S, Shimoda K, Takenaga Y (2015) Loss of ADAR1 in human iPS cells promotes caspase3-mediated apoptotic cell death. Genes Cells 20(8):675–680. https://doi.org/10.1111/gtc.12261
Kong L, Wu Q, Zhao L, Ye J, Li N, Yang H (2019) Identification of messenger and long noncoding RNAs associated with gallbladder cancer via gene expression profile analysis. J Cell Biochem 120(12):19377–19387. https://doi.org/10.1002/jcb.28953
Kuang WB, Deng Q, Deng CT et al (2018) MiRNA regulates OCT4 expression in breast cancer cells. Eur Rev Med Pharmacol Sci. 22(5):1351–1357. https://doi.org/10.26355/eurrev_201803_14478
Kurozumi S, Yamaguchi Y, Kurosumi M, Ohira M, Matsumoto H, Horiguchi J (2017) Recent trends in microRNA research into breast cancer with particular focus on the associations between microRNAs and intrinsic subtypes. J Hum Genet 62(1):15–24. https://doi.org/10.1038/jhg.2016.89
Li P, Zhou L, Zhao T et al (2017a) Caspase-9: structure, mechanisms and clinical application. Oncotarget. 8(14):23996–24008. https://doi.org/10.18632/oncotarget.15098
Li B, Li X, Xiong H et al (2017b) Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells. Oncotarget. 8(31):51748–51757. https://doi.org/10.18632/oncotarget.18518
Liang T, Guo L, Liu C (2012) Genome-wide analysis of mir-548 gene family reveals evolutionary and functional implications. J Biomed Biotechnol. 2012:679563. https://doi.org/10.1155/2012/679563
Liu B, Li J, Cairns MJ (2014) Identifying miRNAs, targets and functions. Brief Bioinform 15(1):1–19. https://doi.org/10.1093/bib/bbs075
Liu DZ, Chang B, Li XD, Zhang QH, Zou YH (2017) MicroRNA-9 promotes the proliferation, migration, and invasion of breast cancer cells via down-regulating FOXO1. Clin Transl Oncol 19(9):1133–1140. https://doi.org/10.1007/s12094-017-1650-1
Loh HY, Norman BP, Lai KS, Rahman NM, Alitheen NBM, Osman MA (2019) The regulatory role of MicroRNAs in breast cancer. Int J Mol Sci 20(19):4940. https://doi.org/10.3390/ijms20194940
Lu J, Zhang M, Yang X, Cui T, Dai J (2017a) MicroRNA-548c-3p inhibits T98G glioma cell proliferation and migration by downregulating c-Myb. Oncol Lett. 13(5):3866–3872. https://doi.org/10.3892/ol.2017.5870
Lu Y, Qin T, Li J et al (2017b) MicroRNA-140-5p inhibits invasion and angiogenesis through targeting VEGF-A in breast cancer. Cancer Gene Ther 24(9):386–392. https://doi.org/10.1038/cgt.2017.30
Luo Z, Li D, Luo X et al (2016) Decreased expression of miR-548c-3p in osteosarcoma contributes to cell proliferation via targeting ITGAV. Cancer Biother Radiopharm. 31(5):153–158. https://doi.org/10.1089/cbr.2016.1995
Ni XF, Zhao LH, Li G et al (2018) MicroRNA-548-3p and MicroRNA-576-5p enhance the migration and invasion of esophageal squamous cell carcinoma cells via NRIP1 down-regulation. Neoplasma. 65(6):881–887. https://doi.org/10.4149/neo_2018_171206N803
Ren L, Li Y, Zhao Q et al (2020) miR-519 regulates the proliferation of breast cancer cells via targeting human antigen R. Oncol Lett. 19(2):1567–1576. https://doi.org/10.3892/ol.2019.11230
Saffari M, Ghaderian SMH, Omrani MD, Afsharpad M, Shankaie K, Samadaian N (2019) The association of miR-let 7b and miR-548 with PTEN in prostate cancer. Urol J. 16(3):267–273. https://doi.org/10.22037/uj.v0i0.4564
Serra KP, Peres RM, Sarian LO et al (2016) Cyclooxygenase-2 (COX2) and p53 protein expression are interdependent in breast cancer but not associated with clinico-pathological surrogate subtypes, tumor aggressiveness and patient survival. Acta Histochem 118(2):176–182. https://doi.org/10.1016/j.acthis.2015.12.009
Shi Y, Qiu M, Wu Y, Hai L (2015) MiR-548c-3p functions as an anti-oncogenic regulator in breast cancer. Biomed Pharmacother 75:111–116. https://doi.org/10.1016/j.biopha.2015.07.027
Shi W, Bruce J, Lee M et al (2016) MiR-449a promotes breast cancer progression by targeting CRIP2. Oncotarget. 7(14):18906–18918. https://doi.org/10.18632/oncotarget.7753
Tormo E, Pineda B, Serna E et al (2015) MicroRNA profile in response to doxorubicin treatment in breast cancer. J Cell Biochem 116(9):2061–2073. https://doi.org/10.1002/jcb.25162
Wang T, Du M, Zhang W et al (2019) MicroRNA-432 suppresses invasion and migration via E2F3 in nasopharyngeal carcinoma. Onco Targets Ther. 12:11271–11280. https://doi.org/10.2147/ott.s233435
Wei YT, Guo DW, Hou XZ, Jiang DQ (2017) miRNA-223 suppresses FOXO1 and functions as a potential tumor marker in breast cancer. Cell Mol Biol 63(5):113–118. https://doi.org/10.14715/cmb/2017.63.5.21
Wu X, Chen S, Lu C (2020) Amyloid precursor protein promotes the migration and invasion of breast cancer cells by regulating the MAPK signaling pathway. Int J Mol Med 45(1):162–174. https://doi.org/10.3892/ijmm.2019.4404
Zendjabil M, Favard S, Tse C, Abbou O, Hainque B (2017) Les microRNA comme biomarqueurs: quelles perspectives. C R Biol. 340(2):114–131. https://doi.org/10.1016/j.crvi.2016.12.001
Zubor P, Kubatka P, Dankova Z et al (2018) miRNA in a multiomic context for diagnosis, treatment monitoring and personalized management of metastatic breast cancer. Future Oncol. 14(18):1847–1867. https://doi.org/10.2217/fon-2018-0061
Funding
Tuo Xin Project. Research Funding of First Teaching Hospital of Tianjin University of Traditional Chinese Medicine (No. 201902).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflicts of interest
The authors declare that there are no conflicts of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Tan, PY., Wen, LJ., Li, HN. et al. MiR-548c-3p inhibits the proliferation, migration and invasion of human breast cancer cell by targeting E2F3. Cytotechnology 72, 751–761 (2020). https://doi.org/10.1007/s10616-020-00418-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10616-020-00418-3