Abstract
Enhanced oxidative stress plays an important role in the progression and onset of diabetes and its complications. Strategies or efforts meant to reduce the oxidative stress are needed which may mitigate these pathogenic processes. The present study aims to investigate the in vitro ameliorative potential of nine antioxidant molecules in L6 myotubes under oxidative stress condition induced by 4-hydroxy-2-nonenal and also to comprehend the gene expression patterns of oxidative stress genes upon the supplementation of different antioxidants in induced stress condition. The study results demonstrated a marked increase in the level of malondialdehyde and protein carbonyl content with a subsequent increase in the free radicals that was reversed by the pretreatment of different dietary antioxidant. From the expression analysis of the oxidative stress genes, it is evident that the expression of these genes is modulated by the presence of antioxidants. The highest expression was found in the cells treated with Insulin in conjugation with an antioxidant. Resveratrol is the most potent modulator followed by Mangiferin, Estragole, and Capsaicin. This comparative analysis ascertains the potency of Resveratrol along with Insulin in scavenging the reactive oxygen species (ROS) generated under induced stress conditions through antioxidant defense mechanism against excessive ROS production, contributing to the prevention of oxidative damage in L6 myotubes.
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Acknowledgements
The authors are grateful to Department of Biotechnology, Government of India for the research grant (BT/362/NE/TBP/2012) extended towards completion of this work. The authors thank Dr. Bidyut Kumar Sharma, Director, DBT-AAU Centre, Assam Agricultural University for providing instrumental support. The authors also thank Gunajit Goswami, Research Scholar, Assam agricultural University for extending his help in executing this research work. The authors would like to thank Prof. S.S. Ghosh and Anil Bidkar from IIT Guwahati for the help extended in the study.
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PS and AB performed the experimental work, and compilation of data. PS drafted the manuscript. SB designed the study, facilitated infrastructural and financial support to carry out the experiments. All authors read and approved the final manuscript.
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Purabi Sarkar and Ananya Bhowmick are contributed equally to this work.
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Fig. 1
a–b Activities of SOD, CAT in control and treatment groups of L6 myotubes. Results are expressed as means and standard deviations of the control and treated cells from triplicate measurements (n = 3) of three biological replicates. Data were subjected to one-way ANOVA and the significance of differences between means was calculated by Tukey’s Multiple Comparison Test using Graph pad Prism Software and significance was accepted at P < 0.05. *P < 0.05; **P < 0.01; ***P < 0.001 versus control and #P < 0.05; ##P < 0.01; ###P < 0.001 versus HNE treated (DOC 549 kb)
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Sarkar, P., Bhowmick, A. & Banu, S. Comparative analysis of different dietary antioxidants on oxidative stress pathway genes in L6 myotubes under oxidative stress. Cytotechnology 70, 1177–1192 (2018). https://doi.org/10.1007/s10616-018-0209-5
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DOI: https://doi.org/10.1007/s10616-018-0209-5