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Involvement of 15-lipoxygenase-1 in the regulation of breast cancer cell death induced by sodium butyrate

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Abstract

15-Lipoxygenase-1 (15-Lox-1) as a member of fatty acid dioxygenases family has received considerable attention as an effector of cancer cell growth. The relevance of sodium butyrate on 15-Lox-1 pathway has not been determined in breast cancer. This study is aimed to investigate the possible involvement of 15-Lox-1 in the regulation of breast cancer cell growth by sodium butyrate. MTT assay was used to assess the cytotoxicity effect and Annexin-V-FITC staining was applied for detection of apoptosis using flow cytometry. The involvement of 15-Lox-1 was examined using 15-Lox-1 specific inhibitor and enzyme gene expression level and activity was further analyzed by Real-time PCR and measurement of 13(S)-HODE. The results revealed that sodium butyrate increased the expression of 15-Lox-1 and production of 13(S)HODE. 15-Lox-1 was also involved in the sodium butyrate-induced breast cancer cell cytotoxicity and apoptosis. This study provided more evidences on the positive effectiveness of 15-Lox-1/13(S)-HODE on controlling growth of breast cancer cells.

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Acknowledgments

This work was financially supported by Iran University of Medical Sciences (Grant Number: 93-01-30-24624).

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Authors and Affiliations

  1. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Vahid Salimi

  2. Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mohammad Shabani, Mitra Nourbakhsh & Masoumeh Tavakoli-Yaraki

  3. Metabolic Disorders Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mitra Nourbakhsh

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  1. Vahid Salimi
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  2. Mohammad Shabani
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  4. Masoumeh Tavakoli-Yaraki
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Correspondence to Masoumeh Tavakoli-Yaraki.

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Salimi, V., Shabani, M., Nourbakhsh, M. et al. Involvement of 15-lipoxygenase-1 in the regulation of breast cancer cell death induced by sodium butyrate. Cytotechnology 68, 2519–2528 (2016). https://doi.org/10.1007/s10616-016-9972-3

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