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Responses of primary cultured haemocytes derived from the marine gastropod Haliotis tuberculata to an industrial effluent exposure

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Abstract

This study assessed the responses of primary cultured haemocytes from the marine gastropod Haliotis tuberculata exposed to the increasing concentrations of industrial effluent (0, 0.5, 1, 10, 15 and 20%) discharged into the Tunisian coastal area. Analyses showed the presence of metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), zinc (Zn), nickel (Ni) and lead (Pb) in the effluent. The effects of this mixture of pollutants on abalone haemocyte parameters were reflected by a significant decrease of cell viability, phagocytotic activity and reactive oxygen species (ROS) production as well as morphological and lysosomal membrane alterations. Thus, these results indicated that our primary culture system represents a suitable in vitro model for monitoring of anthropogenic contaminants in aquatic environments.

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Acknowledgements

This work was supported by a grant from the Ministry of higher education and scientific research, University of Sfax, Tunisia to R. Ladhar-Chaabouni. The authors thank L. Poulain and M. Duval (Plateau Technique de Cytométrieen Flux,Université de Caen Normandie, France) for their helpful technical assistance, the technical staff of the Centre de Recherche en Environnement Côtier (Luc-sur-Mer, Normandie, France) for their assistance in animal care and F. Sdirat for English revision.

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Authors and Affiliations

  1. Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia

    Rim Ladhar-Chaabouni, Sahar Karray & Amel Hamza-Chaffai

  2. Normandie université, 14032, Caen, France

    Thomas Houel, Antoine Serpentini & Jean-Marc Lebel

  3. UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, 14032, Caen Cedex 5, France

    Thomas Houel, Antoine Serpentini & Jean-Marc Lebel

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  1. Rim Ladhar-Chaabouni
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  2. Thomas Houel
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  3. Antoine Serpentini
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  4. Sahar Karray
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  5. Jean-Marc Lebel
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  6. Amel Hamza-Chaffai
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Correspondence to Rim Ladhar-Chaabouni.

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Ladhar-Chaabouni, R., Houel, T., Serpentini, A. et al. Responses of primary cultured haemocytes derived from the marine gastropod Haliotis tuberculata to an industrial effluent exposure. Cytotechnology 69, 191–200 (2017). https://doi.org/10.1007/s10616-016-0050-7

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  • DOI: https://doi.org/10.1007/s10616-016-0050-7

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