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Investigation of relationship between IL-6 gene variants and hypertension in Turkish population

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Abstract

Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a polymorphic gene which −174 G/C is a common and −572 G/C is a rare polymorphisms identified in promoter region. Publications on IL-6 gene polymorphisms raised the question whether this gene polymorphisms lead to susceptibility to HT or not. To investigate the effects of IL-6 gene −174 G/C (rs 1800795) and −572 G/C (rs1800796) polymorphisms on plasma IL-6 and CRP levels and their associations with hypertension disease in Turkish population we analyzed −174 G/C and −572 G/C polymorphisms and plasma IL-6 and CRP levels in 111 healthy controls and 108 hypertension patients from Adıyaman, Turkey. We determined the genotypes using polymerase chain reaction-restriction fragment length polymorphism and analyzed plasma levels of IL-6 by ELISA and CRP by automated standard biochemical methods. We have found no statistically significant differences between IL-6 gene −174 G/C and −572 G/C genotypes and allelic frequencies and IL-6 and CRP plasma levels and HT (p > 0.05). No CC genotype was found in control subjects for −572 G/C polymorphism. In conclusion, we found relation to −174 G/C and −572 G/C gene variants between neither IL-6 and CRP levels nor hypertension. The −572 G allele and GG genotype are predominant in Turkish population in Adıyaman, Turkey whereas the CC genotype is very rare.

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Acknowledgments

This study was supported by a grant of the Research Foundation of Adıyaman University (FEFYL/2012-0002), Turkey.

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The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Biology, Science and Art Faculty, Adıyaman University, Adıyaman, Turkey

    Esin Karaman, Meral Urhan Kucuk & Yunus Kucukkaya

  2. Department of Medical Biology, Faculty of Medicine, Mustafa Kemal University, 31024, Antakya, Hatay, Turkey

    Meral Urhan Kucuk

  3. Department of Biology, Faculty of Science and Art, Artvin Coruh University, Artvin, Turkey

    Aysegul Bayramoglu & Halil Ibrahim Guler

  4. Department of Biophysics, Faculty of Medicine, Mustafa Kemal University, Antakya, Turkey

    Semire Uzun Göçmen

  5. Department of Cardiology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey

    Süleyman Ercan

  6. Department of Medical Documentation and Secretarial, Vocational School of Health Services, Mersin University, Mersin, Turkey

    Sema Erden

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  1. Esin Karaman
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  2. Meral Urhan Kucuk
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Correspondence to Meral Urhan Kucuk.

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Karaman, E., Urhan Kucuk, M., Bayramoglu, A. et al. Investigation of relationship between IL-6 gene variants and hypertension in Turkish population. Cytotechnology 67, 947–954 (2015). https://doi.org/10.1007/s10616-014-9732-1

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