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Human adipose tissue derived mesenchymal stem cells are resistant to several chemotherapeutic agents

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Abstract

Human adipose derived mesenchymal stem cells (ADMSCs) are multipotential stem cells, originated from the vascular stromal compartment of fat tissues which can be used as an alternative cell source for many different cell therapies. However, their response to chemotherapeutic agants remains unknown. Here we assessed the acute direct effects of individual chemotherapeutic drug on ADMSCs. Using an in vitro culture system, the response of ADMSCs to the three chemotherapeutic agents cisplatin, comptothecin and vincristine was determined in comparison with that of testicular germ cell tumour (TGCT) cell line. The recovery of cell numbers following exposure to chemotherapeutic agents were also evaluated. Our results showed that human ADMSCs were resistant to chemo-therapeutic agents which are commonly used in clinic, the full recovery was seen respectively in ADMSCs after the drug treatment. Moreover, ADMSCs maintained their stem cell characteristics in vitro after the exposure to all chemotherapeutic agents.

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Abbreviations

ADMSC:

Adipose derived mesenchymal stem cell

MSCs:

Mesenchymal stem cells

XTT:

2,3-Bis(2-methoxy-4-nitro-5-sulphophenyl)-5-((phenylamino)carbonyl)2H-tetrazolium hydroxide

FITC:

Fluoresein-5-isothiocyanate

FACS:

Fluorescence-activated cell sorting

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Acknowledgments

This work was supported by grants from the “863 Projects” of Minsitry of Science and Technology of PR China (No 2002 AA 205061); and from Beijing Minsitry of Science and Technology (No. 2002-489).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Oncology, Center for Cell Therapy and Tissue Engineering, The First Affiliated Hospital of Anhui Medical University, 218# Jixi Road, Hefei, 230022, Anhui Province, People’s Republic of China

    Wei Liang & Hailong Xia

  2. Institute of Basic Medical Science and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, People’s Republic of China

    Jing Li & Robert Chunhua Zhao

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  1. Wei Liang
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  2. Hailong Xia
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  3. Jing Li
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  4. Robert Chunhua Zhao
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Correspondence to Wei Liang.

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Liang, W., Xia, H., Li, J. et al. Human adipose tissue derived mesenchymal stem cells are resistant to several chemotherapeutic agents. Cytotechnology 63, 523–530 (2011). https://doi.org/10.1007/s10616-011-9374-5

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  • DOI: https://doi.org/10.1007/s10616-011-9374-5

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