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Isolation and characterization of eight polymorphic microsatellite loci for the Mediterranean gorgonian Paramuricea clavata

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Abstract

The gorgonian Paramuricea clavata is a benthic organism often included in conservation management plans since it creates complexity in the ecosystems and is extremely vulnerable to disturbances. Eight microsatellite markers isolated from an enriched genomic library were characterized in a total of 50 individuals from two north-western Mediterranean populations. All loci were polymorphic and the number of alleles per locus ranged between 2 and 14. Significant genetic differentiation was observed between populations. The polymorphic markers presently isolated will allow for the assessment of the spatial genetic structure between and within populations of this umbrella species of the Mediterranean Sea.

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Acknowledgments

We thank I. Calderón, A. Blanquer and people from L. Serra’s laboratory for technical support and useful comments, and R. Coma and E. Macpherson for collecting samples. This research was supported by projects 119/2003 and CTM2007-66635 (CICYT) from the Spanish Government.

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Correspondence to Gemma Agell.

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Agell, G., Rius, M. & Pascual, M. Isolation and characterization of eight polymorphic microsatellite loci for the Mediterranean gorgonian Paramuricea clavata . Conserv Genet 10, 2025–2027 (2009). https://doi.org/10.1007/s10592-009-9885-1

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  • DOI: https://doi.org/10.1007/s10592-009-9885-1

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