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Development of microsatellite markers for Polemonium kiushianum (Polemoniaceae), a critically endangered grassland plant species in Japan

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Abstract

Eight microsatellite loci were isolated and characterized for Polemonium kiushianum (Polemoniaceae), an endangered perennial herb endemic to the Kyushu region in Japan. The number of individuals of this species is presumed to be about 400 individuals. The loci showed polymorphisms with two to ten alleles per locus. The expected and observed heterozygositis ranged from 0.039 to 0.744 and 0.039 to 0.784, respectively. Although significant deviations (P < 0.05) from Hardy–Weinberg equilibrium were detected for one locus, no significant deviations were detected for other seven loci. There was no evidence of significant linkage disequilibrium between any two of the loci. The markers described here will be useful to establish conservation programs based on genetic traits of P. kiushianum.

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References

  • Environment Agency of Japan (2000) Threatened wildlife of Japan–red data book–vol 8, vascular plants (ed. Center JWR). Japan Wildlife Research Center, Tokyo

    Google Scholar 

  • Goudet J (1995) FSTAT (Version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486

    Google Scholar 

  • Lian C, Hogetsu T (2002) Development of microsatellite markers in black locust (Robinia pseudoacasia) using a dual-suppression-PCR technique. Mol Ecol Notes 2:211–213. doi:10.1046/j.1471-8286.2002.00213.x

    Article  CAS  Google Scholar 

  • Lian C, Wadud MA, Geng Q, Shimatani K, Hougetsu T (2006) An improved technique for isolating codominant compound microsatellite markers. J Plant Res 119:415–417. doi:10.1007/s10265-006-0274-2

    Article  CAS  PubMed  Google Scholar 

  • Milligan B (1992) Plant DNA isolation. In: Hoelzel AR (ed) Molecular genetic analysis of populations: a practical approach. IRL Press, Oxford, pp 59–88

    Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

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Acknowledgments

We thank S. Sei, S. Nagahara, Y. Takahashi, N. Fujii and T. Abe for their helpful support of sampling. This work was supported by Grants in Aid for Scientific Research (20241056) from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Graduate School of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto, 606-8502, Japan

    Masashi Yokogawa, Shingo Kaneko & Yuji Isagi

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  1. Masashi Yokogawa
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  2. Shingo Kaneko
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  3. Yuji Isagi
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Correspondence to Masashi Yokogawa.

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Yokogawa, M., Kaneko, S. & Isagi, Y. Development of microsatellite markers for Polemonium kiushianum (Polemoniaceae), a critically endangered grassland plant species in Japan. Conserv Genet 10, 1445 (2009). https://doi.org/10.1007/s10592-008-9757-0

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  • DOI: https://doi.org/10.1007/s10592-008-9757-0

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