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Development of microsatellite loci for the black-footed limpet, Patella depressa, and cross-amplification in two other Patella species

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Abstract

Limpets (Patella spp.) are suitable organisms to investigate the effects of climate change in marine systems. They are widespread over NE Atlantic intertidal rocky shores and have been extensively studied in terms of population dynamics and ecology. Within genus Patella, microsatellites have only been developed for Patella caerulea and cross-species tests are unknown. In this work, we describe 11 primer pairs for Patella depressa and the results of cross-species testing on Patella candei and Patella rustica.

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Acknowledgements

This research was supported in part by Fundação para a Ciência e a Tecnologia (FCT), Project number POCTI/BSE/42003/2001, and by Universidad de Vigo. MB and PR were supported by FCT grants SFRH/BPD/5610/2001 and SFRH/BD/8232/2002, respectively, and MP and AL were supported by a Xunta de Galicia and Universidad de Vigo grants, respectively. The authors thank A Seoane, T Silva, P Jara and J Melo-Ferreira for technical assistance in cloning.

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Authors and Affiliations

  1. Campus Agrário de Vairão, CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, 4485–661, Vairão, Portugal

    M. Pérez, M. Branco, P. A. Ribeiro, A. M. Santos & P. Alexandrino

  2. Department of Biochemistry, Genetics and Immunology, University of Vigo, 36310, Vigo, Spain

    A. Llavona & P. Presa

  3. University of Southampton, Southampton, England

    P. A. Ribeiro & S. J. Hawkins

  4. Departamento de Zoologia-Antrologia, Faculdade de Ciências da, Universidade do Porto, 4099–002, Porto, Portugal

    A. M. Santos & P. Alexandrino

  5. Marine Biological Association, Plymouth, England

    S. J. Hawkins

  6. Instituto de Investigación en Recursos Cinegéticos, Ciudad Real, Spain

    J. A. Dávila

Authors
  1. M. Pérez
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  2. M. Branco
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  3. A. Llavona
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  4. P. A. Ribeiro
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  5. A. M. Santos
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  6. S. J. Hawkins
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  7. J. A. Dávila
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  8. P. Presa
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  9. P. Alexandrino
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Corresponding author

Correspondence to P. Alexandrino.

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Pérez, M., Branco, M., Llavona, A. et al. Development of microsatellite loci for the black-footed limpet, Patella depressa, and cross-amplification in two other Patella species. Conserv Genet 8, 739–742 (2007). https://doi.org/10.1007/s10592-006-9195-9

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  • DOI: https://doi.org/10.1007/s10592-006-9195-9

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