Abstract
The maize B chromosome typically undergoes nondisjunction during the second microspore division. For normal A chromosomes, the r-X1 deficiency in maize can induce nondisjunction during the second megaspore and first microspore divisions. However, it is not known whether the r-X1 deficiency also induces nondisjunction of the maize B chromosome during these cell divisions. To answer this question, chromosome numbers were determined in the progeny of r-X1/R-r female parents carrying two B chromosomes. Some of the r-X1-lacking progeny (21.2%) contained zero or two B chromosomes. However, a much higher percentage of the r-X1-containing progeny (43.4%) exhibited zero or two B chromosomes, but none displayed more than two B chromosomes. Thus, the results indicated that the r-X1 deficiency could also induce nondisjunction of the B chromosome during the second megaspore division; moreover, the B chromosome in itself could undergo nondisjunction during the same division. In addition, pollen grains from plants with two B chromosomes lacking or exhibiting the r-X1 deficiency were compared via pollen fluorescence in situ hybridization (FISH) using a B chromosome-specific probe. The results revealed that the r-X1 deficiency could induce the occurrence of B chromosome nondisjunction during the first microspore division and that the B chromosome in itself could undergo nondisjunction during the same division at a lower frequency. Our data shed more light on the behavior of the maize B chromosome during cell division.
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Abbreviations
- DAPI:
-
4′,6-Diamidino-2-phenylindole
- FISH:
-
Fluorescence in situ hybridization
- FITC:
-
Fluorescein isothiocyanate
- RFLP:
-
Restriction fragment length polymorphism
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This work was supported by grants from the Ministry of Science and Technology, Taiwan (MOST 104-2311-B-005-012-MY3).
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Supplementary Fig. S1
The three types of maize ears used in this study. (a) A self-pollinated r-X1/R-r ear, in which the self-colored kernels lack the r-X1 deficiency, and the mottled kernels carry this deficiency. (b) A self-pollinated r/r ear. (c) An F1 ear from a cross of r-X1/R-r plants as the female parents with r/r males, whose colorless kernels harbor the r-X1 deficiency (GIF 436 kb)
Supplementary Fig. S2
Root-tip chromosomes of the inbred W22 line containing two B chromosomes. Arrows denote the B chromosome; the bar represents 10 μm (GIF 211 kb)
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Tseng, SH., Peng, SF. & Cheng, YM. Analysis of B chromosome nondisjunction induced by the r-X1 deficiency in maize. Chromosome Res 26, 153–162 (2018). https://doi.org/10.1007/s10577-017-9567-7
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DOI: https://doi.org/10.1007/s10577-017-9567-7