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Anti-apoptotic Effect of Taxodione on Serum/Glucose Deprivation-Induced PC12 Cells Death

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Abstract

Taxodione, a diterpenoid from the roots of Salvia chorassanica Bunge, possesses cytotoxic, apoptotic, and antimicrobial activity. This study was designed to investigate the protective effects of taxodione on serum/glucose deprivation-induced ischemic injury in PC12 cells and related mechanisms. In an in vitro model of ischemia, PC12 cells were exposed to serum and glucose deprivation for 6 and 18 h. The protective effects of the methanol extract of S. chorassanica and taxodione were assessed using alamarBlue® assay. Intracellular ROS production was measured by fluorimetry using 2′,7′-dichlorofluorescin diacetate (DCFH-DA). The levels of PARP, Bcl-2, and Bax proteins were detected after western blot analysis. It was shown that taxodione (0.2–1.5 μM) significantly increased cell viability in a dose-dependent manner after ischemic insult. Taxodione has antioxidant activity and protects PC12 cells against oxidative stress-induced apoptotic cell death. Meanwhile, pretreatment with taxodione significantly induced an increase in Bcl-2 and a decrease in Bax protein level. The results of this study confirmed the protective effect of taxodione in serum/glucose deprivation-induced ischemic injury and the putative role of apoptosis as a underling mechanisms. Thus, it would be fair to consider taxodione as a promising ingredient of S. chorassanica for the expansion on novel class of anti-ischemic agents.

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Acknowledgments

This work was supported by Grants (No. 900996) from the Research Affairs of the Mashhad University of Medical Sciences as a part of a Pharm.D. thesis. We are also grateful for the editorial assistance of Mrs. Z. Gonzalez.

Conflict of interest

There was no conflict of interest with other people or company.

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Authors and Affiliations

  1. Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, P.O. Box 9188617871, Mashhad, Iran

    Negar Shafaei-Bajestani & Zahra Tayarani-Najaran

  2. Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Seyed Ahmad Emami & Javad Asili

Authors
  1. Negar Shafaei-Bajestani
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  2. Seyed Ahmad Emami
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  3. Javad Asili
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  4. Zahra Tayarani-Najaran
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Correspondence to Zahra Tayarani-Najaran.

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Shafaei-Bajestani, N., Emami, S.A., Asili, J. et al. Anti-apoptotic Effect of Taxodione on Serum/Glucose Deprivation-Induced PC12 Cells Death. Cell Mol Neurobiol 34, 1103–1109 (2014). https://doi.org/10.1007/s10571-014-0085-2

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  • DOI: https://doi.org/10.1007/s10571-014-0085-2

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