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Upregulated Expression of SSTR1 is Involved in Neuronal Apoptosis and is Coupled to the Reduction of bcl-2 Following Intracerebral Hemorrhage in Adult Rats

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Abstract

Somatostatins are peptide hormones that regulate diverse cellular processes, such as neurotransmission, cell proliferation, apoptosis, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. SSTR1 is a member of the superfamily of somatostatin receptors possessing seven-transmembrane segments. Aberrant expression of SSTR1 has been implicated in several human diseases, including pseudotumor cerebri, and oncogenic osteomalacia. In this study, we investigated a potential role of SSTR1 in the regulation of neuronal apoptosis in the course of intracerebral hemorrhage (ICH). A rat ICH model in the caudate putamen was established and subjected to behavioral tests. Western blot and immunohistochemistry indicated a remarkable up-regulation of SSTR1 expression surrounding the hematoma after ICH. Double-labeled immunofluorescence showed that SSTR1 was mostly co-localized with neurons, and was rarely distributed in activated astrocytes and microglia. Additionally, SSTR1 co-localized with active-caspase-3 and bcl-2 around the hematoma. The expression of active-caspase-3 was parallel with that of SSTR1 in a time-dependent manner. In addition, SSTR1 knockdown specifically resulted in reduced neuronal apoptosis in PC12 cells. All our findings suggested that up-regulated SSTR1 contributed to neuronal apoptosis after ICH, which was accompanied with reduced expression of bcl-2.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No. 81371367, No. 31370803), Natural Science Foundation of Jiangsu Province (No. BK2009156, No. BK2009157, No. BK2009161), Nantong City Social Development Projects Fund (No. HS2012032), Development Fund for Collaborative Innovation Center of Glycoscience of Shandong University and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Immunology, Medical College, Nantong University, Nantong, 226001, People’s Republic of China

    Damin Yuan & Yuhong Ji

  2. Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, People’s Republic of China

    Jianhong Shen, Yaohua Yan, Xinmin Wu, Aihong Li, Aisong Guo & Yuanyuan Wu

  3. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, People’s Republic of China

    Chengwei Duan

  4. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, People’s Republic of China

    Jiabing Shen

  5. Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, 226001, Jiangsu Province, People’s Republic of China

    Cuiying Tang

  6. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, People’s Republic of China

    Dongmei Zhang

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  1. Damin Yuan
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  3. Yaohua Yan
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  9. Jiabing Shen
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  10. Cuiying Tang
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  11. Dongmei Zhang
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  12. Yuhong Ji
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Correspondence to Dongmei Zhang or Yuhong Ji.

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Damin Yuan and Janhong Shen contributed equally to this work.

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Yuan, D., Shen, J., Yan, Y. et al. Upregulated Expression of SSTR1 is Involved in Neuronal Apoptosis and is Coupled to the Reduction of bcl-2 Following Intracerebral Hemorrhage in Adult Rats. Cell Mol Neurobiol 34, 951–961 (2014). https://doi.org/10.1007/s10571-014-0081-6

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  • DOI: https://doi.org/10.1007/s10571-014-0081-6

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