Abstract
Mangiferin has been extensively applied in different fields due to its anti-inflammatory properties. However, the precise mechanism used by mangiferin on lipopolysaccharide (LPS)-induced inflammation has not been elucidated. Here, we discuss the potential mechanism of mangiferin during a LPS-induced brain injury. Brain injury was induced in ICR mice via intraperitoneal LPS injection (5 mg/kg). Open- and closed-field tests were used to detect the behaviors of mice, while immunoblotting was performed to measure the expression of interleukin-6 (IL-6) and cystathionine-b-synthase (CBS) in the hippocampus after mangiferin was orally administered (p.o.). Mangiferin relieved LPS-induced sickness 6 and 24 h after LPS injection; in addition, this compound suppressed LPS-induced IL-6 production after 24 h of LPS induction as well as the downregulation of LPS-induced CBS expression after 6 and 24 h of LPS treatment in the hippocampus. Therefore, mangiferin attenuated sickness behavior by regulating the expression of IL-6 and CBS.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31100762, 81371300), the Education Departmental Natural Science Research Funds of Jiangsu Provincial Higher School of China (11KJB310013, 13KJB310021), the Key Laboratory of Brain Disease Bioinformation of Jiangsu Province (Jsbl1102), the Foundation of President of Xuzhou Medical College (2012KJZ06, 2011KJ11), and the Qing Lan Project of Jiangsu Province.
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Yan-Yan Fu, Fang Zhang, and Lei Zhang contributed equally to this work.
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Fu, YY., Zhang, F., Zhang, L. et al. Mangiferin Regulates Interleukin-6 and Cystathionine-b-Synthase in Lipopolysaccharide-Induced Brain Injury. Cell Mol Neurobiol 34, 651–657 (2014). https://doi.org/10.1007/s10571-014-0039-8
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DOI: https://doi.org/10.1007/s10571-014-0039-8