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Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Gerbil Septum Submitted to Various Durations of Cerebral Ischemia

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Abstract

The extent of neuronal damage/death in some brain regions is highly correlated to duration time of transient ischemia. In the present study, we carried out neuronal degeneration/death and glial changes in the septum 4 days after 5, 10, 15, and 20 min of transient cerebral ischemia using gerbils. To examine neuronal damage, Fluoro-Jade B (F-J B, a marker for neuronal degeneration) histofluorescence staining was used. F-J B positive (+) cells were detected in the septo-hippocampal nucleus (SHN) of the septum only in the 20 min ischemia-group; the mean number of F-J B+ neurons was 14.9 ± 2.5/400 μm2 in a section. Gliosis of astrocytes and microglia was examined using anti-glial fibrillary acidic protein (GFAP) and anti-ionized calcium-binding adapter molecule 1 (Iba-1), respectively. In all the ischemia-groups, GFAP- and Iba-1-immunoreactive astrocytes and microglia, respectively, were increased in number, and apparently tended to be increased in their immunoreactivity. Especially, in the 20 min ischemia-group, the number and immunoreactivity of Iba-immunoreactive microglia was highest and strongest in the ischemic SHN 4 days after ischemia–reperfusion. In brief, our findings showed that neuronal damage/death in the SHN occurred and gliosis was apparently increased in the 20 min ischemia-group at 4 days after ischemia–reperfusion.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012R1A1A2001404), and by 2012 Kangwon National University Hospital Grant.

Conflict of interest

The authors have declared that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Emergency Medicine, and Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Chan Woo Park & Jun Hwi Cho

  2. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Jae-Chul Lee, Ji Hyeon Ahn, Joon Ha Park, In Hye Kim & Moo-Ho Won

  3. Laboratory of Neuroscience, Department of Physical Therapy, College of Rehabilitation Science, Daegu University, Gyeongsan, 712-714, South Korea

    Dae Hwan Lee

  4. Department of Neuroscience, College of Medicine, Korea University, Seoul, 136-705, South Korea

    Geum-Sil Cho

  5. Institute of Integrative Traditional & Western Medicine, Medical College, Yangzhou University, Yangzhou, 225001, China

    Bing Chun Yan

  6. Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Hui Young Lee

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  1. Chan Woo Park
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Correspondence to Moo-Ho Won or Jun Hwi Cho.

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Chan Woo Park and Jae-Chul Lee contributed equally to this article.

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Park, C.W., Lee, JC., Ahn, J.H. et al. Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Gerbil Septum Submitted to Various Durations of Cerebral Ischemia. Cell Mol Neurobiol 33, 991–1001 (2013). https://doi.org/10.1007/s10571-013-9967-y

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