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Responses to Apical and Basolateral Application of Glutamate in Mouse Fungiform Taste Cells with Action Potentials

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Abstract

In taste bud cells, glutamate may elicit two types of responses, as an umami tastant and as a neurotransmitter. Glutamate applied to apical membrane of taste cells would elicit taste responses whereas glutamate applied to basolateral membrane may act as a neurotransmitter. Using restricted stimulation to apical or basolateral membrane of taste cells, we examined responses of taste cells to glutamate stimulation, separately. Apical application of monosodium glutamate (MSG, 0.3 M) increased firing frequency in some of mouse fungiform taste cells that evoked action potentials. These cells were tested with other basic taste compounds, NaCl (salty), saccharin (sweet), HCl (sour), and quinine (bitter). MSG-sensitive taste cells could be classified into sweet-best (S-type), MSG-best (M-type), and NaCl or other electrolytes-best (N- or E/H-type) cells. Furthermore, S- and M-type could be classified into two sub-types according to the synergistic effect between MSG and inosine-5′-monophosphate (S1, M1 with synergism; S2, M2 without synergism). Basolateral application of glutamate (100 μM) had almost no effect on the mean spontaneous firing rates in taste cells. However, about 10% of taste cells tested showed transient increases in spontaneous firing rates (>mean + 2 standard deviation) after basolateral application of glutamate. These results suggest the existence of multiple types of umami-sensitive taste cells and the existence of glutamate receptor(s) on the basolateral membrane of a subset of taste cells.

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Acknowledgments

This work was supported by KAKENHI 18109013, 18077004 (YN), and 21791808 (RY).

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Authors and Affiliations

  1. Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Mayu Niki, Shingo Takai, Yoko Kusuhara, Yuzo Ninomiya & Ryusuke Yoshida

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  1. Mayu Niki
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  2. Shingo Takai
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  3. Yoko Kusuhara
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  4. Yuzo Ninomiya
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  5. Ryusuke Yoshida
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Correspondence to Ryusuke Yoshida.

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Niki, M., Takai, S., Kusuhara, Y. et al. Responses to Apical and Basolateral Application of Glutamate in Mouse Fungiform Taste Cells with Action Potentials. Cell Mol Neurobiol 31, 1033–1040 (2011). https://doi.org/10.1007/s10571-011-9702-5

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  • DOI: https://doi.org/10.1007/s10571-011-9702-5

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